| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Malignant and Normal Rectum Tissues Using 1H NMR Spectroscopy |
| ZHAO Mei-xian1,2,GAO Xiu-xiang2,QI Jian2,YAO Hong-wei3,LI Hui-zhen2,XU Yi-zhuang2*,TANG Guang-shi1,WANG Jing4,WU Jin-guang2 |
1. Department of Applied Chemistry, College of Science, Beijing University of Chemical Technology, Beijing 100029, China
2. College of Chemistry and Molecular Engineering, Peking University, Institute of Inorganic Chemistry, Beijing 100871, China
3. Third Hospital, Peking University, Beijing 100083, China
4. Research Institute of Petroleum Processing, Beijing 100083, China |
|
|
|
|
Abstract Cancer is one of the most serious diseases, a threat to human body’s health and a causes of death. The early diagnosis of cancer and timely therapy is significant for improving the survival. Owing to the complexity and limit of conventional medical diagnosis, misdiagnosis of ten occurs in many cases. NMR spectroscopy is an effective technique for characterizing molecular structure and component changes. The component and structural information of nucleic acid, protein, lipid and glucide in the biologic tissues can also be distinguished from NMR data. In the present paper, a new method was developed for the diagnosis of tumor using the advanced physical chemistry (NMR) and biomedicine technique. Nine rectum tissue samples and their corresponding normal tissues of rectum were measured using NMR spectroscopy. Each tissue sample obtained from the operation was quickly separated into two parts averagely: one was dipped into 10% formalin solution and prepared for conventional pathological examination; the other was preserved in liquid nitrogen for further NMR detection. Before NMR detection, the corresponding sample was thawed at room temperature and was dipped into 0.5 mL D2O. Dioxane was used as external reference. The obtained NMR spectra were analyzed and compared by OMNIC5.0TM and SPSS 11.0TM Software. The differences of metabolite in the tissues samples were studied and a method for the diagnosis of cancer using NMR data was initially explored. The result indicates that the 1H NMR spectra of rectum cancer tissues are significantly different from those of their corresponding normal rectum tissues. This is shown by the differences in the integral area ratio at characteristic peak region. A0.9/A3.0, A1.3/A3.0, A2.0/A3.0, A1.3/A0.9 and A4.1/A3.0 of the normal rectum tissues are higher than those of their corresponding cancer tissues, but A3.2/A3.0 is lower than that of the rectum cancer tissues. From the above result it is concluded that the concentration of fatty acid, inositol and lactate is lower in the rectum cancer tissues than in the normal tissues, while that of choline bases compounds is higher. The NMR judgments were also consistent with the pathological examination results. So the NMR spectra may be developed into a method of early diagnosing rectum cancer through these differences.
|
|
Received: 2006-11-05
Accepted: 2007-02-08
|
|
|
|
Corresponding Authors:
XU Yi-zhuang
E-mail: xyz@pku.edu.cn
|
|
[1] Mountford Carolyn E, Doran Sinead, Lean Cynthia L, et al. Chemical Reviews,2004, 104: 3677.
[2] Podo F. NMR Biomed., 1999, 12(7): 413.
[3] CHEN Wen-xue, DENG Feng, YUE Yong(陈文学, 邓 风, 岳 勇). Chinese Journal of Magnetic Resonance(波谱学杂志),2004,21(1): 127.
[4] Howe Franklyn A, Kirstie S Opstad. NMR Biomed.,2003, 16: 123.
[5] Millis Kevin, Weybright Patrick, Campbell Natalee, et al. Magnetic Resonance in Medicine, 1999, 41: 257.
[6] WANG Mei-hao, LI Jian-ce(王美豪, 李建策). Journal of Wenzhou Medical College(温州医学院学报),2001, 31(5): 338.
[7] Martínez-Bisbal Carmen M, Martí-Bonmati Luis, José Piquer, et al. NMR Biomed.,2004, 17: 191.
[8] Payne G S, Troy H, Vaidya S J, et al. NMR Biomed.,2006, 19: 593.
[9] Franks Suzanne E, Smith Mitchell R, Arias-Mendoza Fernando, et al. Leukemia Research,2002,26: 919.
[10] Valonen Piia K, Griffin Julian L. NMR Biomed.,2005, 18: 252.
[11] FANG Hong, GUO Qing-lin, ZHANG Xiang, et al(方 虹, 郭庆林, 章 翔, 等). Journal of The Fourth Military Medical University(第四军医大学学报),2001,22(8): 729.
[12] Valonen Piia K, Griffin Julian L. NMR Biomed.,2005, 18: 252.
[13] Sitter Beather, Ursula Sonnewald, Spraul Manfred, et al. NMR Biomed., 2002, 15: 327.
[14] Mahon Marrita M, deSouza Nandita M, Dina Roberto, et al. NMR Biomed.,2004, 17: 144.
[15] Govindaraju V, Young K, Maudsley A A. NMR Biomed.,2000, 13: 129.
[16] XU Yi-zhuang, ZHAO Ying, XU Zhi, et al(徐怡庄, 赵 莹, 徐 智, 等) . Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(11): 1775.
[17] LING Xiao-feng, LI Wei-hong, SONG Yuan-yuan, et al(凌晓锋, 李维红, 宋苑苑, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000,20(5): 692.
[18] WANG Fan, LING Xiao-feng, YANG Zhan-lan, et al(王 凡，凌晓峰，杨展澜, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(3): 498.
[19] REN Yu, XU Yi-zhuang, WANG Jing, et al(任 予，徐怡庄，王 晶, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(4): 681.
[20] YANG Li-min, XU Zhi, ZHANG Yuan-fu, et al(杨丽敏，徐 智，张元福, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(5): 883.
[21] SUN Xue-jun, LI Qing-bo, SHI Jing-sen, et al(孙学军，李庆波，石景森, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(8): 933.
[22] ZHAO Yuan-li, Lü Jing, GE Xiang-hong, et al(赵元黎, 吕 晶, 葛向红, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(7): 1267. |
| [1] |
LIU Fei1, TAN Jia-jin1*, XIE Gu-ai2, SU Jun3, YE Jian-ren1. Early Diagnosis of Pine Wilt Disease Based on Hyperspectral Data and Needle Resistivity[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3280-3285. |
| [2] |
LIANG Wan-jie1, FENG Hui2, JIANG Dong3, ZHANG Wen-yu1, 4, CAO Jing1, CAO Hong-xin1*. Early Recognition of Sclerotinia Stem Rot on Oilseed Rape by Hyperspectral Imaging Combined With Deep Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2220-2225. |
| [3] |
LI Xiang, LI Zhong-feng, GAO Jun, WANG Xia, ZHANG Xin*, ZHANG Zhuo-yong*. Analysis of Metabolism During Mung Bean Germination by 1H-NMR Combining With MCR-ALS With Selectivity Constraint[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3010-3014. |
| [4] |
LIU Dong1，SONG Bin1, SUN Xue-jun2*, XU Yi-zhuang3*, LONG Yan-bin1, DU Jun-kai2, ZHENG Jian-bao2, LIU Bin1, DUAN Xiang-long1, WANG Jian-hua1, LIU Si-da1, MAO Zhi-jun1, ZHANG Yuan-fu3, WU Jin-guang3. Research of Fourier Transform Infrared (FTIR) Spectroscopy with Multivariate Analysis as Novel Diagnostic Tool for Metastatic Rectal Cancer Lymph Nodes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2028-2031. |
| [5] |
WANG Wei, PAN Zhi-feng*, TANG Wei-yue, LI Yun-tao, FAN Chun-zhen . Research on Early Diagnosis of Gastric Cancer by the Surface Enhanced Raman Spectroscopy of Human Hemoglobin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3402-3405. |
| [6] |
ZHOU Xue1, CHEN Guang-yi2, ZHANG Jian-min1, FAN Chun-zhen1, LIANG Er-jun1, GUO Yan2, TANG Wei-yue1* . Research on Early Diagnosis of Esophageal Cancer by Raman Spectroscopy of Human Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(11): 2989-2992. |
| [7] |
LI Xiao-long1, MA Zhan-hong1, ZHAO Long-lian2, LI Jun-hui2, WANG Hai-guang1* . Early Diagnosis of Wheat Stripe Rust and Wheat Leaf Rust Using Near Infrared Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(10): 2661-2665. |
| [8] |
RUAN Sen-lin1，2, WANG Ru1, CHEN Xiao-yan1, WU Ya-ying1, LIU Qing1, LIU Min1, RAO Cui1, DENG Hong1* . Initial Research on FTIR Spectroscopy of Lyophilized Serum from Colorectal Cancer Patients[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(02): 354-358. |
| [9] |
ZHU Jing-ping1,ZHANG Yun-yao1,LI Yan1,HU Hao-tun2,LI Bin1,XIANG Rong1 . FTIR Spectroscopic Investigation of Normal and Malignant Pleomorphic Adenoma [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 2947-2950. |
| [10] |
GAO Xiu-xiang1,YAO Hong-wei2,DU Jun-kai3,ZHAO Mei-xian1, 4,QI Jian1,LI Hui-zhen1, 5,PAN Qing-hua1, 6, XU Yi-zhuang1*,WU Jin-guang1. Study on Malignant and Normal Rectum Tissues Using IR and 1H and 31P NMR Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(04): 969-973. |
| [11] |
GAO Xiu-xiang1, HE Wen-yi2, YAO Hong-wei3, DU Jun-kai4, ZHAO Mei-xian1, 5, QI Jian1, LI Hui-zhen1, 6, XU Yi-zhuang1*, WU Jin-guang1. Study on the Malignant and Normal Rectum Tissues Using NMR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2201-2206. |
| [12] |
ZHU Dian-ming1,2,JIN Wan-xiang1,LUO Xiao-sen2,LIU Ying2,SHEN Zhong-hua2,LU Jian2,NI Xiao-wu2*. Identification of Protoporphyrin IX Fluorescence Spectrum in Human Blood Serum by Biorthogonal Spline Wavelet[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(08): 1879-1882. |
| [13] |
LIU Hua-qing,JIANG Feng-chi. Investigation on the Change in C—F Stretching Vibration of FTIR Spectrum by 1H NMR Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2243-2245. |
| [14] |
MENG Tao1,3,XU Duan-fu1,ZHAO Ying1,WANG Du-jin1,XU Yi-zhuang2*,QI Jian2,WU Jin-guang2 . New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2069-2072. |
| [15] |
MENG Tao1,XU Duan-fu1,XU Yi-zhuang2,ZHAO Ying1,WANG Du-jin1,WU Jin-guang2* . Investigation on the Fluorescence Spectroscopy of Normal and Malignant Rectum Tissues[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(06): 1156-1160. |
|
|
|
|
