| 光谱学与光谱分析 |
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| The Secondary Structure of Heated Whey Protein and Its Hydrolysates Antigenicity |
| PANG Zhi-hua1,2, ZHU Jun1,2, WU Wei-jing1,2, WANG Fang1,2, REN Fa-zheng1,2, ZHANG Lu-da3, GUO Hui-yuan1,2* |
1. College of Food Science & Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China
2. Beijing Higher Leaning Institution Engineering Research Center of Animal Product, Beijing 100083, China
3. College of Science, China Agricultural University, Beijing 100094, China |
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Abstract Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) were used to investigate the conformational changes of heated whey protein (WP) and the corresponding changes in the hydrolysates immunoreactivity were determined by competitive enzyme-linked immunosorbent assay (ELISA). Results showed that the contents of α- helix and β-sheet of WP did not decrease much under mild heating conditions and the antigenicity was relatively high; when the heating intensity increased (70 ℃ for 25 min or 75 ℃ for 20 min), the content of α- helix and β-sheet decreased to the minimum, so was the antigenicity; However, when the WP was heated at even higher temperature and for a longer time, the β-sheet associated with protein aggregation begun to increase and the antigenicity increased correspondingly. It was concluded that the conformations of heated WP and the antigenicity of its hydrolysates are related and the optimum structure for decreasing the hydrolysates antigeniity is the least content of α-helix and β-sheet. Establishing the relationship between the WP secondary structure and WP hydrolysates antigenicity is significant to supply the reference for antigenicity reduction by enzymolysis.
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Received: 2010-10-26
Accepted: 2011-01-20
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Corresponding Authors:
GUO Hui-yuan
E-mail: guohuiyuan99@gmail.com
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[1] Hst A, Halken S. Allergy, 1998，(53): 111.
[2] Wal J M. Allergy, 2001，(56): 35.
[3] Su R, Qi W, He Z, et al. Food Hydrocolloids, 2008，(22)：995.
[4] Schmidt D G, Meijer R J G M, Slangen C J, et al. Clinical and Experimental Allergy, 1995，(25): 1007.
[5] Kananen A, Savolainen J, Mkinen J, et al. International Dairy Journal, 2000，(10): 691.
[6] Kim S B, Ki K, Khan S M A, et al. Journal of Dairy Science, 2007，(90): 4043.
[7] Pelton J T, McLean L R. Analytical Biochemistry, 2000，(277): 167.
[8] Ngarize S, Herman H, Adams A, et al. Journal of Agricultural and Food Chemistry, 2004，(52): 6470.
[9] LU Yan, ZHANG Wei-wei, WANG Gong-ke (卢 雁，张玮玮，王公轲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008，28(1): 88.
[10] Wada R, Fujita Y, Kitabatake N. Biochimica et Biophysica Acta, 2006，(1760): 841.
[11] Jedrychowski L, Wróblewska B. Food and Agricultural Immunology, 1999，(11): 91.
[12] Matheus S, Friess W, Mahler H C. Pharmaceutical Research, 2006，(23): 1350.
[13] Matsudomi N, Oshita T, Kobayashi K. Journal of Dairy Science, 1994，(77): 1487.
[14] Matsuura J E, Manning M C. Journal of Agricultural and Food Chemistry, 1994，(42): 1650.
[15] Zhu H M, Damodaran S. Journal of Agricultural and Food Chemistry, 1994，(42): 846.
[16] Roefs S P F M, De Kruif K G. European Journal of Biochemistry, 1994，(226): 883.
[17] Morisawa Y, Kitamura A, Ujiharaw T, et al. Clinical and Experimental Allergy, 2009，(39): 918.
[18] Kleber N, Krause I, Illgner S, et al. European Food Resarch and Technology, 2004，(219): 105. |
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