| 光谱学与光谱分析 |
|
|
|
|
|
| Raman Spectra and Structure Analysis of 2,6-Pyridine Dicarboxylic Acid in Different States and Single Bacillus Spore |
| HUANG Rong-shao1, HUANG Xi1, 2, XU Lan-lan2, 3, LI Yong-qing4, HUANG Shu-shi2, 3* |
1. College of Agronomy, Guangxi University, Nanning 530004, China
2. Lab of Biophysics, Guangxi Academy of Sciences, Nanning 530003, China
3. Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650224, China
4. Department of Physics, East Carolina University, Greenville, NC 27858-4353, USA |
|
|
|
|
Abstract The Raman spectra of 2,6-pyridine dicarboxylic acid (DPA) and their calcium salts(Ca-DPA) in different states and the Ca-DPA in a single bacterial spore have been recorded by Laser Tweezers Raman system (LTRS) and the spectra have been assigned. Raman spectra of different states of DPA and Ca-DPA are different evidently. Analysis leading to differences in the structure of spectrum may be due to that the Raman spectra of DPA crystalline reflected more precise characteristics information compared to DPA powder, in which the laser can penetrate through DPA crystalline and the Raman scatter from the crystalline interior is greater than that from DPA powder. The second reason is that DPA powder and Ca-DPA crystalline contain water molecules, and the intermolecular hydrogen bonding in the crystals of these molecules is extensive. The presence of calcium ions would affect the pyridine ring so that both sides of the carboxyl pyridine ring have a certain geometric deformation and the hydroxy carboxylic was damaged. The DPA2-anion is principal in Ca-DPA and the DPA solution. The calcium ion affects the stability of the pyridine ring structure in the Ca-DPA solution. The result from the spectra also showed that the DPA in single spores present Ca-DPA crystal state.
|
|
Received: 2010-05-10
Accepted: 2010-08-20
|
|
|
|
Corresponding Authors:
HUANG Shu-shi
E-mail: hshushi@gxas.cn, hshushi@yahoo.com.cn
|
|
[1] Setlow B, Atluri S, Kitchel R, et al. Journal of Bacteriology, 2006, 188： 3740.
[2] Takusagawa F, Hirotsu K, Shimada A. Bull. Chem. Soc. Jpn., 1973, 46: 2020.
[3] Telléz V C, Gaytán B S, Bernès S, et al. Acta Crystallogr. C, 2002, 58: 0228.
[4] Strahs G, Dickerson R E. Acta Crystallogr. B, 1968,24: 571.
[5] Carmona P. Spectrochim. Acta A, 1980, 36: 705.
[6] Scully M, Kattawar G M, Lucht R P, et al. PNAS, 2002, 99: 10994.
[7] Kolomenskii A A, Jerebtsov S N, Opatrny T, et al. J. Mod. Phys., 2003, 50: 2369.
[8] Ghiamati E, Manoharan R, W.H. Nelson W H, et al. Appl. Spectrosc., 1992, 46: 357.
[9] Phillips T, Sample J L, Scholl P F, et al. Mater. Res. Soc. Symp. Proc., 2003, 738: G8.7.1.
[10] Petry R, Schmit M, Popp J. Chem. Physchem., 2003, 4(1): 14.
[11] Xie C A, Li Y Q. Journal of Applied Physics, 2003, 93: 2982.
[12] Xie C A, Mace J, DinnoM A, et al. Anal. Chem., 2005, 77: 4390.
[13] Ojeda J F, Xie C A, Li Y Q, et al. Optics Express, 2006, 14(12): 5385.
[14] Chen D, Huang S S, L i Y Q. Anal. Chem., 2006, 78: 6936.
[15] McCann K, Laane J. Journal of Molecular Structure, 2008, 890: 346.
[16] Kolomenskii A A, Schuessler H A. Spectrochimica Acta Part A, 2005, 61: 647.
[17] Manoharan R, Ghiamati E, Dalterio R A, et al. Appl. Spectroscopy，1993,47： 2145.
[18] Huang S S, Chen D, Pelczar P L, et al. J. Bacteriol., 2007, 189(13): 4681.
[19] LEI Wei，HE Xin-sheng(雷 桅, 贺新生). Journal of Southwest University of Science and Technology(西南科技大学学报), 2009, 19(4): 90. |
| [1] |
QIAN Duo, SU Wen-en, LIU Zhi-yuan, GAO Xiao-yu, YI Yu-xin, HU Cong-cong, LIU Bin, YANG Sheng-yuan*. Soy Protein Gold Nanocluster as an “Off-On” Fluorescent Probe for the Detection of Bacillus Anthracis Biomarkers DPA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1815-1820. |
| [2] |
LI Zong-xiang1, 2, ZHANG Ming-qian1*, YANG Zhi-bin1, DING Cong1, LIU Yu1, HUANG Ge1. Application of FTIR and XRD in Coal Structural Analysis of Fault
Tectonic[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 657-664. |
| [3] |
YU Chun-mei, ZHANG Nan, TENG Hai-peng. Investigation of Different Structures of Coals Through FTIR and Raman Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(07): 2050-2056. |
| [4] |
XIANG Ying1, FENG An-di2, ZHAO Zheng-zhuo1, SUN Cong-dan1, XU Hao-peng1, SUN Li-na1, XIE Feng-ying1, 3*. Infrared Spectrum and Thermodynamic Studies of Catechin-Black Rice Anthocyanin Complex[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3416-3419. |
| [5] |
XIE Feng-ying1, LI Feng-feng1, ZHANG Shuang1, BI Wei-wei2, ZHANG Xiu-ling1*, ZHANG Xiao-nan1. Analysis of Acylation Modification of Black Rice Anthocyanins Using Fourier Transform Infrared Spectroscopy (FTIR)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2386-2389. |
| [6] |
ZHANG Ying, YANG Shu-ming* . The Research Development of FTIR in Analyzing Material Structures [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 54-57. |
| [7] |
SHANG Jian-li, ZHANG Hao*, XIONG Lei, MA Xiang-long . Structural Analysis and Optimization Performance Forecast of SiO2-Based Phase Change and Humidity Storage Composite Materials with Fourier Transform Infrared Spectrum [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(08): 2430-2436. |
| [8] |
MA Shu-rong1, ZHANG Hai-shan1*, ZHOU Chang-yu1, CHEN Lei2, YU Zhi2, WANG Bing2, ZHAO Bing2* . Surface-Enhanced Raman Spectroscopy of Thiuram Pesticide [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(10): 2683-2687. |
| [9] |
WANG Xue1, 2, SUN Mei-juan1, 2, LIU Jun-xian1, DENG Yang-ge1, MO Yu-xiang1, TAO Zhan-hua2* . Analysis of Astaxanthin in Phaffia Rhodozyma Using Laser Tweezers Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(09): 2433-2437. |
| [10] |
ZHENG Huai-li，FANG Hui-li，JIANG Shao-jie，YANG Chun，MA Jiang-ya，ZHANG Zhao-qing . Preparation and Structural Analysis of Diatomite-Supported SPFS Flocculant[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(07): 1917-1921. |
| [11] |
WANG Yi-bin1, 2, MIAO Jin-lai1, 2*, HE Bi-juan3, LIANG Qiang1, 2, LIU Fang-ming1, 2, ZHENG Zhou1, 2 . Laser Tweezers Raman Spectroscopy Analysis of Cold-Adapted Aromatic Hydrocarbons-Degradating Strains Isolated from Antarctic Sea [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(02): 418-421. |
| [12] |
LIU Hao1,XIANG Bing-ren2*,QU Ling-bo3,XU Jian-ping2 . Structure Analysis of Benzoic Medicines by Near Infrared and Two Dimensional Correlation Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(02): 265-269. |
| [13] |
YU Chun-ri1,2, CUI Zhi-feng2,WANG Rong-kai3 . The Analysis of Vibrational Structure of the Electronic Spectra of Diatomic Molecules[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(06): 1012-1017. |
|
|
|
|
