| 光谱学与光谱分析 |
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| Fluorescence Fluctuation Spectroscopy with Strong Background Fluorescence——An Monte Carlo Approach |
| LIU Guang, CHEN Bo, MENG Fan-bo, MA Hui*, CHEN Die-yan |
| Key Laboratory for Atomic and Molecular NanoSciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084, China |
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Abstract Fluorescence fluctuation spectroscopy is a method in which fluorescence fluctuations arising from a very small sample volume are analysed to obtain brightness, diffusion coefficient and concentration of particles. Using Monte Carlo simulation, the influences of background autofluorescence and noises on fluorescence fluctuation spectroscopy are studied. Results show that a two- component photon counting histogram can effectively remove the effects due to background autofluorescence from low brightness and high concentration components and uniform noise. The result will help to bring applications of fluorescence fluctuation spectroscopy to intracellular environment.
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Received: 2003-02-08
Accepted: 2003-04-28
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Corresponding Authors:
MA Hui
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| Cite this article: |
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LIU Guang,CHEN Bo,MENG Fan-bo, et al. Fluorescence Fluctuation Spectroscopy with Strong Background Fluorescence——An Monte Carlo Approach[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1379-1383.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I11/1379 |
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