光谱学与光谱分析 |
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Study on Optimizing the Analysis Condition for Active Compound in Eucommia Olive |
PENG Mi-jun1,2,ZHOU Chun-shan2*,LIU Jian-lan1,ZHANG Min1,LEI Qi-fu2 |
1. Jishou University, Zhangjiajie 427000, China 2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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Abstract The absorption spectroscopic determination of aucubin by condensation with p-dimethylaminobenzaldehyde was optimized in this paper. A high sensitive method was established and the linearity range of the method was from 4.5 to 45 μg·mL-1. The influences of the factors, such as the dosage of coloring agent, reaction time, heating temperature and the dose of acid etc., on the system were studied. And the result indicated that all of the factors had obvious effects on the system. The method has been applied to determining the content of aucubin in the extract of Eucommia olive with satisfactory results.
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Received: 2003-09-06
Accepted: 2003-12-26
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Corresponding Authors:
ZHOU Chun-shan
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Cite this article: |
PENG Mi-jun,ZHOU Chun-shan,LIU Jian-lan, et al. Study on Optimizing the Analysis Condition for Active Compound in Eucommia Olive [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1655-1658.
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URL: |
https://www.gpxygpfx.com/EN/Y2004/V24/I12/1655 |
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