光谱学与光谱分析 |
|
|
|
|
|
Determination of Polyphenols in Tea by a New Method Spectrophotometry |
ZHANG Shu-fang |
College of Chemistry, Qufu Normal University, Qufu 273165, China |
|
|
Abstract The authors developed a method for the determination of tea polyphenols. Phenanthroline is a sensitive regent for the determination of Fe2+, it can react with Fe2+ to form an orange complex in acidic solution. A new spectrophotometric method was proposed for the determination of tea polyphenols in tea samples based on that tea polyphenols could react with Fe3+ to produce Fe2+,and the orange complex would be formed by the reaction of Fe2+ with Phenanthroline in acidic solution. In the presence of positive iron surfactant CTMAB, the sensitivity of chromogenic reaction was improved.The work focused on the optimizing of experiment conditions such as wavelength of determination, buffer solution, surfactant, and the react time etc. At the maximum absorption wavelength of 510 nm, the apparent molar absorptivity was 0.88×105 L·mol-1·cm-1,Beer’s law was obeyed in the range of 4.00 to 32.00 μg·mL-1 Polyphenols. The detection limit was 2.0 μg·mL-1. This method was applied to the determination of polyphenols in tea samples with satisfactory results. The method has three merits, namely high level of accuracy and sensitivity, simplicity, and simple equipment.
|
Received: 2007-03-28
Accepted: 2007-06-29
|
|
Corresponding Authors:
ZHANG Shu-fang
E-mail: zhshf999@sina.com
|
|
[1] LI Xiao-li, HE Yong, QIU Zheng-jun(李晓丽, 何 勇,裘正军). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007,27(2):279. [2] YANG Mei-yan,LI Nan, XING Guo-xiu, et al(杨美燕, 李 楠,邢国秀, 等). China New Medicine(中国新医药), 2003, 2(6):50. [3] LIN Yang-sheng, WU Sang-shung, LIN Jen-kun. J. Agric. Food Chem., 2003, 51:975. [4] Hiroyuki Sakakibara, Yoshinori Honda, Satoshi Nakagawa, et al. J. Agric. Food Chem., 2003, 51:571. [5] Daniel J Zeeb, Bryant C Nelson, Klaus Albert, et al. Anal. Chem., 2000, 72:5020. [6] ZHU Qin-yan, CHEN Zhen-yu(朱勤艳,陈振宇). Chinese Journal of Analysis Laboratory(分析试验室), 1999, 18(4):70. [7] TANG Gen-yuan, WU Hong-jing, WU Ling(唐根源,吴红京,吴 棱). Chinese Journal of Chromatography(色谱), 2001, 19(3):233. [8] TANG Sen-fu(唐森富). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2007, 24(2):113. [9] YU Yu-yan,SHA Zheng, ZHANG Shu-ling, et al(余宇燕,沙 政,张淑玲, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2006,25(4):101. [10] WANG Lin, WANG Jiong(王 琳,王 炯). Physical Testing and Chemical Analysis, Part B:Chemical Analysis(理化检验·化学分册), 2005, 41(11):738. [11] SUN Yao-guo,LIN Min, L Jin, et al(孙耀国, 林 敏,吕 进, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2004, 21(5):940. [12] CAO Yu-hua, ZHANG Xin, DING Xiang-huan, et al(曹玉华,张 欣,丁祥欢, 等). Chinese Journal of Analytical Chemistry(分析化学), 2001, 29(9):1072. [13] GB 8313—83. Tea(茶叶):Determination of Tea(茶多酚的测定). [14] WANG Li-zhu, WU Leng, YAO Yuan-gen, et al(王丽珠,吴 棱,姚元根, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 1997, 14(3):52. [15] LUO Yi-fan, GUO Zhen-fei, ZHU Zhen-yu, et al(罗一帆, 郭振飞,朱振宇, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8):1230. [16] CHEN Hua-cai, L Jin, FENG Chun-hong, et al(陈华才,吕 进,俸春红, 等). Journal of China Institute of Metrology(中国计量学院学报), 2005, 16(1):17. [17] LANG Hui-yun,LIAO Xiao-ling, DU Yue-qin(郎惠云, 廖晓玲,杜月琴). Journal of Analytical Science(分析科学学报), 2003, 19(2):151. [18] LIAO Xiao-ling,LANG Hui-yun, XU Wen-feng, et al(廖晓玲, 朗惠云,徐文峰, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2003, 22(5):70. [19] HUI Rui-hua, HOU Dong-yan, GUAN Chong-xin, et al(回瑞华,侯冬岩,关崇新, 等). Journal of Instrumental Analysis(分析测试学报), 2003, 22(6):60. [20] Lü Yan-yang, CAO Shu-qin(吕艳阳, 曹书勤). Chinese Journal of Chemical Regent(化学试剂),2005,27(2):101. |
[1] |
ZHANG Rong1, 2, DUAN Ning1, 3, JIANG Lin-hua1, 3*, XU Fu-yuan3, JIN Wei3, LI Jian-hui1. Study on Optical Path Optimization for Direct Determination of
Spectrophotometry of High Concentration Hexavalent Chromium
Solution by Ultraviolet Visible Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1829-1837. |
[2] |
WANG Qiu, LI Bin, HAN Zhao-yang, ZHAN Chao-hui, LIAO Jun, LIU Yan-de*. Research on Anthracnose Grade of Camellia Oleifera Based on the Combined LIBS and Fourier Transform NIR Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1450-1458. |
[3] |
LI Xiao1, CHEN Yong2, MEI Wu-jun3*, WU Xiao-hong2*, FENG Ya-jie1, WU Bin4. Classification of Tea Varieties Using Fuzzy Covariance Learning
Vector Quantization[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 638-643. |
[4] |
SUN Xu-dong1, 2, LIAO Qi-cheng1, HAN Xi3, Hasan Aydin4, XIE Dong-fu1, GONG Zhi-yuan1, FU Wei1, WANG Xin-peng1. Research on Online Detection of Tea Stalks and Insect Foreign Bodies by Near-Infrared Spectroscopy and Fluorescence Image Combined With
Electromagnetic Vibration Feeding[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 100-106. |
[5] |
LIU Meng-xuan1, 2, 3, 4, WU Qiong5, WANG Xu-quan1, 2, 4, CHEN Qi5, ZHANG Yong-gang1, 2, HUANG Song-lei1, 2*, FANG Jia-xiong1, 2*. Validity and Redundancy of Spectral Data in the Detection Algorithm of Sucrose-Doped Content in Tea[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3647-3652. |
[6] |
YAO Shan1, ZHANG Xuan-ling1, CAI Yu-xin1, HE Lian-qiong1, LI Jia-tong1, WANG Xiao-long1, LIU Ying1, 2*. Study on Distribution Characteristics of Different Nitrogen and
Phosphorus Fractions by Spectrophotometry in Baiyangdian
Lake and Source Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1306-1312. |
[7] |
LIU Qin-rong1, DU Zi-wei1, LI Jia-zhen1, WANG Yi-shuo1, 3*, GU Xuan2, CUI Xiu-mei2. Analysis and Evaluation of Inorganic Elements in Salvia miltiorrhiza and Rhizosphere Soils From Different Areas[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3618-3624. |
[8] |
SUN Xu-dong, LIU Jun-bin. THz Spectroscopy Detection of Insect Foreign Body Hidden in Tea Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2723-2728. |
[9] |
WU Xian-xue1, 2, LI Ming2, LI Liang-xing2, DENG Xiu-juan1, MA Xian-ying3, LI Ya-li1, ZHOU Hong-jie1*. Study on the Homogeneity of Tea Powder by Infrared Spectral Similarity Evaluation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1417-1423. |
[10] |
YANG Bao-hua1, GAO Yuan1, WANG Meng-xuan1, QI Lin1, NING Jing-ming2. Estimation Model of Polyphenols Content in Yellow Tea Based on Spectral-Spatial Features[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 936-942. |
[11] |
WU Bin1*, FU Hai-jun2, WU Xiao-hong2, 3*, CHEN Yong2, JIA Hong-wen1. Classification of FTNIR Spectra of Tea via Possibilistic Fuzzy Discriminant C-Means Clustering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(02): 512-516. |
[12] |
FU Hai-jun1, 2, ZHOU Shu-bin1, 3, WU Xiao-hong1, 2*, WU Bin4, SUN Jun1, 2, DAI Chun-xia1, 5. Mixed Fuzzy Maximum Entropy Clustering Analysis of FT-NIR Spectra of Tea[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(11): 3465-3469. |
[13] |
CAI Qing-kong1, LI Er-jun2, JIANG Jin-bao3*, QIAO Xiao-jun3, JIANG Rui-bo1, FENG Hai-kuan4, 5, 6, LIU Shao-tang1, CUI Xi-min3. Study on the Tea Identification of Near-Infrared Hyperspectral Image Combining Spectra-Spatial Information[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(08): 2522-2527. |
[14] |
LI Zhen-xia1, SHEN Huan-huan1, GAO Miao-miao1, QI Jiao1, LI Qing-yan2*. Analysis of Lycopene in Different Solvents by Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(04): 1114-1117. |
[15] |
YANG Ze-ming1, 2, LI Cai1*, LU Gui-xin1, CAO Wen-xi1. A Study of Fast Detection of Nitrite in Seawater Based on Sequential Injection Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 589-595. |
|
|
|
|