光谱学与光谱分析 |
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Fluorescence Analysis of Trace Glucose Using Glucose Oxidase and Horseradish Peroxidase |
LIU Qing-ye1, LIANG Yue-yuan1, LIANG Ai-hui2, JIANG Zhi-liang1, 2* |
1. School of Environment and Resource, Guangxi Normal University, Guilin 541004, China 2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China |
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Abstract In acetate buffer solution and in the presence of glucose oxidase (GOD), glucose reduced the dissolved oxygen to form H2O2 that oxidized catalytically the excess KI to from I-3 by horseradish peroxidase (HRP). The I-3 combines respectively with rhodamine S (RhS), rhodamine 6G(Rh6G), butyl-rhodamine B(b-RhB) and rhodamine B(RhB) to form RhS-I3, Rh6G-I3, b-RhB-I3 and RhB-I3 associated particles that result in fluorescence quenching at 556, 556, 584 and 584 nm, respectively. Under the optimal conditions, the concentration of glucose in the range of 0.083-9.99, 0.17-8.33, 0.33-8.33 and 0.33-9.99 μmol·L-1 is linear with their fluorescence quenching at 556, 556, 584 and 584 nm, with detection limits of 0.059,0.17,0.21 and 0.16 μmol·L-1 glucose. And the regression equation was ΔF=40.0c+3.0, ΔF=23.9c+8.1, ΔF=25.6c+4.2, and ΔF=18.4c+0.8, respectively. The RhS system was the most sensitive and stable, and was chosen for use. Influence of some foreign substances on the RhS fluorescence quenching determination of 6.67 μmol·L-1 glucose was examined, with a relative error of ±10%. Results showed that 1 000-fold Mg2+ and Cu2+, 300-fold Mn2+,100-fold Zn2+, Al3+ and Co2+, 60-fold L-tyrosine, urea and nicotinic acid, 50-fold Fe3+, HSA and BSA, 10-fold sucrose, vitamin B2, L-lysine, L-glutamic acid and L-cystine did not interfere with the determination. This RhS fluorescence quenching assay was applied to the determination of glucose in the serum samples with satisfactory results.
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Received: 2008-04-12
Accepted: 2008-07-16
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Corresponding Authors:
JIANG Zhi-liang
E-mail: zljiang@mailbox.gxnu.edu.cn
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[1] Kang X H, Mai Z B, Zou X Y, et al. Anal. Biochem., 2007, 369, 71. [2] Oliveira A C A, Assis V C, Matos A C, et al. Anal. Chim. Acta, 2005, 535: 213. [3] Maria C G, Townsend A. Anal. Chim. Acta, 1992, 261: 137. [4] Yuan J P, Guo W W, Wang E K. Biosensors and Bioelectronics, 2008, 23: 1567. [5] Marquette C A, Blum L J. Sensors and Actuators B-Chemical, 2003, 90: 112. [6] Li Z M, Zhu G H, Liu J M, et al. Spectrochim Acta Part A, 2007, 67: 1153. [7] Song S, Sun L, Yuan L, et al. J. Chromatograph A, 2008, 1179: 125. [8] Andreescu S, Linda A L. Anal. Biochem., 2008, 375: 282. [9] Dai Z H, Bao J C, Yang X D, et al. Biosensors and Bioelectronics, 2008, 23: 1070. [10] Cano M A J L, Maye′n M, Mena M L, et al. J. Electroanal Chem., 2008, 615: 69. [11] Jeykumari D R S, Narayanan S S. Biosensors and Bioelectronics, 2008, 23: 1404. [12] Yang Y L, Tseng T F, Yeh J M, et al. Sens. Actuat. B, 2008, 131: 533. [13] LI Yan, YANG Yan-ming, LIU Shu-yuan, et al(李 燕, 杨焱明, 刘树元, 等). Anal. Test Technology Instrument(分析测试技术与仪器), 2007, 13(4): 236. [14] Jiang Z L, Zhou S M, Liang A H, et al. Environ. Sci. Technol., 2006, 40: 4286. [15] Liang A H, Zhou S M, Jiang Z L. Talanta, 2006, 70: 444.
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