| 光谱学与光谱分析 |
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| Studies on the Physiological Response of Phytolacca Americana to Manganese Toxicity by FTIR Spectroscopy |
| REN Li-min1,2,CHENG Ze-feng1,LIU Peng1*,LI Zhi-gang2 |
1. Key Laboratory of Botany,Zhejiang Normal University,Jinhua 321004,China
2. College of Agronomy,Guangxi University,Nanning 530004，China |
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Abstract A technique based on Fourier transform infrared (FTIR) spectrometry was used to detect the physiological changes in chemical composition of Phytolacca americana under a wide range of Mn2+ treatments (0,0.125,0.25,0.5 and 1 g·kg-１). Using the roots,stems and leaves of phytolacca americana,absorption bands corresponding to carbohydrates,ester and proteins varied differently. The absorption band heights at 3 000 and 2 916 cm-1 of stem tissues rose firstly and then decreased,indicating the exudation and transporting situation of organic substances which served as organic osmotic contents to enhance manganese tolerance under low treatments; Meanwhile,the band heights at 2 922 cm-1(roots) and 1 606 cm-1 (leaves) which have different tendency in accordance with Mn treatments showed that the capability to chelate Mn decreased under higher manganese concentration. In addition,the changes of the bands at 1 732 and 1 026 cm-1 (roots),1 028 cm-1 (stems),1 052 and 967 cm-1 (leaves) differed from each other,suggesting that under the conditions of severe Mn stress the ex-oxidation of membrane lipid increased; Otherwise,the band heights at 1 375 cm-1 increased firstly and then decreased,representing that the cell wall improved Mn resistance by increasing cation-exchange capacity(CEC). The result also implied that it is practical to apply FTIR to the research comparing the chemical differences of phytoaccumulators under metal treatments.
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Received: 2006-11-06
Accepted: 2007-02-08
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Corresponding Authors:
LIU Peng
E-mail: pliu99@163.com
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