光谱学与光谱分析 |
|
|
|
|
|
Analysis of Polysaccharides Contents in Ophiopogon Japonicus by NIR |
WANG Yuan1,2,QIN Min-jian1,2*, QI Jin1,2, YU Bo-yang1,2, TANG Li3 |
1. College of Traditional Chinese Medicine, China Pharmaceutical University,Nanjing 210009,China 2. Key Lab of Modern Chinese Medicine, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China 3. Sanjiu Traditional Chinese Medicine Material S&T Co. Ltd., Ya’an 625000, China |
|
|
Abstract In the present paper the polysaccharides in Ophiopogon japonicus was analysed quantitatively by using near infrared spectroscopy. The spectral characteristics of the primary ingredients in ophiopogonis were obtained by applying first derivative, second derivative, smoothing,SNV (standard normal variate), and MSC (multiple scatter correction), and the wavelengths were selected for the best model. Meantime, in combination with the PLS algorithm the calibration process was performed for the quantitative analysis of the polysaccharides in Ophiopogon japonicus. The result showed that after optimizing all the factors, the best model of equation was using “first derivative”+“MSC”+“SG”, and the wavelengths for the best model were selected in 4 000-4 900, 5 100-6 900 and 7 050-10 000 cm-1. The result showed a fine precision of the method, with R2, RMSEC, R2CV, RMSECV and principal factors being 0.996, 0.237, 0.973, 0.583 and 6, respectively. A set of representative samples were used to check the model, and the prediction coefficient of determination was 96.88%.
|
Received: 2008-10-23
Accepted: 2009-01-26
|
|
Corresponding Authors:
QIN Min-jian
E-mail: minjianqin@sina.com
|
|
[1] TIAN You-qing, YU Bo-yang, KOU Jun-ping(田友清, 余伯阳, 寇俊萍). The China Academic Medical Magazine of Organisms(中国医学生物技术应用杂志), 2002, 78(5): 1. [2] FENG Yi, HAN Ning, XU De-sheng(冯 怡,韩 宁,徐德生). Chinese Traditional Patent Medicine(中成药),2006,28(5):705. [3] Blanco M, Villarroya I. Trac-Trends Anal. Chem., 2002, 21(4): 240. [4] Macho S, Rius A, Callao M P, et al. Anal. Chim. Acta, 2001, 445(2): 213. [5] YANG Nan-lin, CHENG Yi-yu, WU Yong-jiang(杨南林,程翼宇,吴永江). Acta Chimica Sinica(化学学报), 2003,61(3): 393. [6] YANG Nan-lin, CHENG Yi-yu, QU Hai-bin(杨南林,程翼宇,瞿海斌). Acta Chimica Sinica(化学学报),2003,61(5): 742. [7] YU Ke, HU Chu-chu, CHENG Yi-yu, et al(虞 科,胡楚楚,程翼宇,等). Chinese Medical Journal(中国药学杂志),2006, 41(3): 226.
|
[1] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
[2] |
LIU Wen-bo, LIU Jin, HAN Tong-shuai*, GE Qing, LIU Rong. Simulation of the Effect of Dermal Thickness on Non-Invasive Blood Glucose Measurement by Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2699-2704. |
[3] |
LIAN Xiao-qin1, 2, CHEN Qun1, 2, TANG Shen-miao1, 2, WU Jing-zhu1, 2, WU Ye-lan1, 2, GAO Chao1, 2. Quantitative Analysis Method of Key Nutrients in Lanzhou Lily Based on NIR and SOM-RBF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2025-2032. |
[4] |
XIE Wen-jing, WANG Jing-jing, LIU Ying, DING Zhou, LIU Ying*. Study on the Fluorescence Emission Characteristics and Change Mechanism of Polysaccharide on White Blood Cell[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1050-1054. |
[5] |
HUANG Wei-bo, CHEN Jia-yun, HUANG Fang, HUANG Li-shan, OUYANG Jian-ming*. Effects of Different Molecular Weight of Gracilaria Lemaneiformis Polysaccharide on Calcium Oxalate Crystal[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1163-1170. |
[6] |
LI Ya-hui1, LI Yan-xiao2*, TAN Wei-long2, SUN Xiao-xia1, SHI Ji-yong1, ZOU Xiao-bo1*, ZHANG Jun-jun1, JIANG Cai-ping1. Rapid Identification and Evaluation of Lycium Ruthenicum Murr. by Near-Infrared and Fourier Transform Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3878-3883. |
[7] |
WANG Peng1,2, SUN Di2, MU Mei-rui3, LIU Hai-xue3, ZHANG Ke-qiang2, MENG Xiang-hui1, YANG Ren-jie1*, ZHAO Run2*. Rapid Detection of Total Nitrogen Through the Manure Movement of in Large-Scale Dairy Farm by Near-Infrared Diffuse Reflectance Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3287-3291. |
[8] |
LI Yun1,2,3, ZHANG Ji1,2, LIU Fei4, XU Fu-rong3, WANG Yuan-zhong1,2*, ZHANG Jin-yu1,2,3*. Prediction of Total Polysaccharides Content in P. notoginseng Using FTIR Combined with SVR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1696-1701. |
[9] |
WU Yong-feng1, DONG Yi-wei1, HU Xin2, Lü Guo-hua1, REN De-chao2, SONG Ji-qing1* . Quantification of Agricultural In-Situ Surface Soil Moisture Content Using Near Infrared Diffuse Reflectance Spectroscopy: A Comparison of Modeling Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3416-3421. |
[10] |
TIAN Bing-mei1, XIE Xiao-mei1,2*, SHEN Pan-pan1, YANG Mo1, ZHANG Sheng-long1, TANG Qing-jiu3 . Preliminary Investigation of the Amount,the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(05): 1331-1334. |
[11] |
ZHAO Feng-chun, WAN Kai-yang, LIU Xiao-qian, ZHANG Qi-wei, GAO Hui-min*, WANG Zhi-min*. Rapid Determination of the Multi-Marker Ingredients in Heterosmilacis Japonicae Rhizoma and Sophorae Flavescentis Radix with Near-Infrared Diffused Reflection Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(10): 2652-2656. |
[12] |
TANG Qi-kun1, WANG Yu1, 2*, WU Yue-jin3, MIN Di1, CHEN Da-wei1, HU Tong-hua1 . Determination of Steviol in Stevia Rebaudiana Leaves by Near Infrared Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(10): 2719-2722. |
[13] |
HU Chao1, 2, BAI Shi-qie1, 2*, ZHANG Yu1, YAN Jia-jun1, YOU Ming-hong1, LI Da-xu1, BAI Ling1, ZHANG Jin1 . Near-Infrared Reflectance Spectroscopy Analytic Model Established for the IVDMD of Cichorium intybus L. [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2085-2088. |
[14] |
ZHANG Yin-tao1, GE Hong-li1*, SUN Zhen1, ZOU Hong-yu2 . Estimating Polysaccharide Content with Hyperspectral in Dendrobium Officinale[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(06): 1645-1648. |
[15] |
JI Peng, WEI Yan-ming*, HUA Yong-li, ZHANG Wen-quan . Discrimination of Polysaccharides from Angelica Sinensis and Its Different Processed Products Based on Fourier Transform Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(05): 1270-1274. |
|
|
|
|