| 光谱学与光谱分析 |
|
|
|
|
|
| Investigation on Damage of Bovine Serum Albumin (BSA) Catalyzed by Nano-Sized Silicon Dioxide (SiO2) under Ultrasonic Irradiation Using Spectral Methods |
| WANG Jun1, 3,DING Na1,ZHANG Zhao-hong2,GUO Ying3,WANG Shi-xian1,XU Rui1,ZHANG Xiang-dong1 |
1. Department of Chemistry, Liaoning University, Shenyang 110036, China
2. Department of Environment, Liaoning University, Shenyang 110036, China
3. Department of Pharmacy, Liaoning University, Shenyang 110036, China |
|
|
|
|
Abstract The damage of bovine serum albumin (BSA) molecules under ultrasonic irradiation in the presence of nano-sized silicon dioxide (SiO2) particles was studied by UV-Vis and fluorescence spectra. In addition, the influences of ultrasonic irradiation time, nano-sized SiO2 addition amount, solution acidity (pH) and ultrasonic irradiation power on the damage of BSA molecules in aqueous solution were also detected. For BSA solution of 1.0×10-5 mol·L-1 at (37.0±0.2) ℃, the UV-Vis spectra of BSA solutions showed that the absorption peaks of BSA displayed obvious hyperchromic effect with the increase in some influence factors such as ultrasonic irradiation time, nano-sized SiO2 addition amount, pH value and ultrasonic irradiation power. However, the fluorescence spectra of BSA solutions showed the phenomenon of fluorescence quenching with the increase in ultrasonic irradiation time, nano-sized SiO2 addition amount, pH value and ultrasonic irradiation power. Moreover, the possible mechanism behind the damage of BSA molecule in the presence of nano-sized SiO2 powders under ultrasonic irradiation was discussed. It was considered that the damage of BSA molecules was attributed to the formation of ·OH radicals resulting from the sonoluminescence and high-heat excitation of ultrasonic cavitation. The research results could be of great significance to using sonocatalytic method to treat tumour in clinic application and for developing nano-sized drug in the future.
|
|
Received: 2007-10-08
Accepted: 2008-01-12
|
|
|
|
Corresponding Authors:
WANG Jun
E-mail: wangjun890@126.com
|
|
[1] Chen L, Rivens I, Terhaar H G, et al. Ultrasound. Med. Biol., 1993, 19(1): 67.
[2] Deep S, Ahluwalia J C. Physical Chemistry and Chemical Physics, 2001, 3(20): 4583.
[3] Vasilescu M, Angelescu D, Almgren M, et al. Langmuir, 1999, 15(8): 2635.
[4] Giancola C, DeSena C, Fessas D, et al. Int. J. Biol. Macromol., 1997, 20(3): 193.
[5] Saboury A A. J. Chem. Thermodyn., 2003, 35(12): 1975.
[6] Eftink M R, Ghivon C A. Anal. Biochem., 1981, 114(2): 199.
[7] WANG Jun, ZHAO Hong-dan, ZHANG Zhao-hong, et al(王 君，赵红丹，张朝红，等). Chinese Journal of Inorganic Chemistry(无机化学学报), 2007, 23(3): 439.
[8] WANG Jun, XU Liang, ZHANG Zhao-hong, et al(王 君，徐 亮，张朝红，等). Acta Chimica Sinica(化学学报), 2005, 63(20): 1921.
[9] WANG Jun, WEN Fu-yu, ZHANG Zhao-hong, et al(王 君，温福宇，张朝红，等). Environmental Science(环境科学), 2006, 27(6): 1134.
[10] Hidaka H, Horikoshi S, Ajisaka K, et al. J. Photochemistry and Photobiology A: Chem., 1997, 108(2-3): 197.
[11] ZHANG Zhao-hong, DONG Dian-bo, ZHAO Zhe, et al(张朝红，董殿波，赵 哲，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(2): 309.
[12] YUE Lin-hai, ZHOU Yong-qiu(岳林海，周永秋). Environmental Pollution & Control (环境污染与防治), 1998, 20(3): 5.
[13] WEI Xiao-fang, DING Xi-ming, LIU Hui-zhou(魏晓芳，丁西明，刘会洲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(4): 556.
[14] Al-Ekabi H, Serpone N. J. Phys. Chem., 1988, 92(20): 5726.
[15] Kruus P, Burk R, Entezari M, et al. Ultrason. Sonochem., 1997, 4(3): 229.
[16] Augugliaro V, Palmisano L, Sclafani A, et al. Toxiological Environ. Chem., 1988, 16(2): 89. |
| [1] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [2] |
DANG Rui, GAO Zi-ang, ZHANG Tong, WANG Jia-xing. Lighting Damage Model of Silk Cultural Relics in Museum Collections Based on Infrared Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3930-3936. |
| [3] |
GUO Wei1, CHANG Hao2*, XU Can3, ZHOU Wei-jing2, YU Cheng-hao1, JI Gang2. Effect of Continuous Laser Irradiation on Scattering Spectrum
Characteristics of GaAs Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3674-3681. |
| [4] |
XU Rong1, AO Dong-mei2*, LI Man-tian1, 2, LIU Sai1, GUO Kun1, HU Ying2, YANG Chun-mei2, XU Chang-qing1. Study on Traditional Chinese Medicine of Lonicera L. Based on Infrared Spectroscopy and Cluster Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3518-3523. |
| [5] |
ZHANG Xiao-dan1, 2, LIU Li-li1*, YU Ying1, CHENG Wei-wei1, XU Bao-cheng1, HE Jia-liang1, CHEN Shu-xing1, 2. Activation of Epigallocatechin Gallate on Alcohol Dehydrogenase:
Multispectroscopy and Molecular Docking Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3622-3628. |
| [6] |
WANG Peng1, GAO Yong-bao1*, KOU Shao-lei1, MEN Qian-ni1, ZHANG Min1, HE Tao1, YAO Wei2, GAO Rui1, GUO Wen-di1, LIU Chang-rui1. Multi-Objective Optimization of AAS Conditions for Determination of Gold Element Based on Gray Correlation Degree-RSM Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3117-3124. |
| [7] |
LIU Pan1, 2, 3, DU Mi-fang1*, LI Bin1, LI Jing-bin1, ZENG Lei1, LIU Guo-yuan1, ZHANG Xin-yao1, 4, ZHA Xiao-qin1, 4. Determination of Trace Tellurium Content in Aluminium Alloy by
Inductively Coupled Plasma-Atomic Emission Spectrometry Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3125-3131. |
| [8] |
LIU Wen-bo, LIU Jin, HAN Tong-shuai*, GE Qing, LIU Rong. Simulation of the Effect of Dermal Thickness on Non-Invasive Blood Glucose Measurement by Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2699-2704. |
| [9] |
WANG Bin1, 2, ZHENG Shao-feng2, GAN Jiu-lin1, LIU Shu3, LI Wei-cai2, YANG Zhong-min1, SONG Wu-yuan4*. Plastic Reference Material (PRM) Combined With Partial Least Square (PLS) in Laser-Induced Breakdown Spectroscopy (LIBS) in the Field of Quantitative Elemental Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2124-2131. |
| [10] |
ZHANG Yue1, 2, LI Yang1, 2, SONG Yue-peng1, 2*. Nondestructive Detection of Slight Mechanical Damage of Apple by Hyperspectral Spectroscopy Based on Stacking Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2272-2277. |
| [11] |
LI Chen-xi1, SUN Ze-yu1, 2, ZHAO Yu2*, YIN Li-hui2, CHEN Wen-liang1, 3, LIU Rong1, 3, XU Ke-xin1, 3. The Research Progress of Two-Dimensional Correlation Spectroscopy and Its Application in Protein Substances Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 1993-2001. |
| [12] |
YUAN Kai-xin, ZHUO Jin, ZHANG Qing-hua, LI Ya-guo*. Study on the Spectral and Laser Damage Resistance of CO2 Laser Modified Sol-Gel SiO2 Thin Films[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1752-1759. |
| [13] |
DING Kun-yan1, HE Chang-tao2, LIU Zhi-gang2*, XIAO Jing1, FENG Guo-ying1, ZHOU Kai-nan3, XIE Na3, HAN Jing-hua1. Research on Particulate Contamination Induced Laser Damage of Optical Material Based on Integrated Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1234-1241. |
| [14] |
HOU Qian-yi1, 2, DONG Zhuang-zhuang1, 2, YUAN Hong-xia1, 2*, LI Qing-shan1, 2*. A Study of the Mechanism of Binding Between Quercetin and CAV-1 Based on Molecular Simulation, Bio-Layer Interferometry and
Multi-Spectroscopy Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 890-896. |
| [15] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
|
|
|
|
