| 光谱学与光谱分析 |
|
|
|
|
|
| Investigation on Fourier Transform Infrared Spectrum of Freshly Resected Cholangiocarcinoma Tissue |
| YIN Gang1,XU Zhi1,XU Yi-zhuang2,SUN Qi-gang1,LIU Ya-qi1,ZHANG Yuan-fu2,WU Jin-guang2,ZHOU Xiao-si1,LING Xiao-feng1* |
1. Department of General Surgery, Peking University Third Hospital, Beijing 100191, China
2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
|
|
|
|
Abstract To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared (FTIR) spectroscopy, 26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery. And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection (ATR) without pretreatment immediately after resection. For each spectrum, 12 bands were identified and assigned between 3 800 and 1 000 cm-1. The peak position was found, and the intensity and area of the bands were measured; The ratios of the intensity and area were calculated. Standard statistic analysis was performed. The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue: 1. The bands related to lipid: The peak of 2 925 cm-1 shifted to lower wave number significantly (P=0.033); 2. The bands related to nucleic acid and lipids: the ratios of I1 083/I1 460(P=0.005), S1 083/S1 460(P=0.001) and S1 240/S1 460(P=0.025) raised significantly, indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy. 3. The bands related to protein: The ratios of I1 550/I1 083(P=0.000)and S1 550/S1 083(P=0.000)reduced significantly, implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably. Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.
|
|
Received: 2009-02-10
Accepted: 2009-05-20
|
|
|
|
Corresponding Authors:
LING Xiao-feng
E-mail: xiaofengling2005@126.com
|
|
[1] Lazaridis K N, Gores G J. Gastroenterology, 2005, 128: 1655.
[2] Olnes M J, Erlich R. Oncology, 2004, 66: 167.
[3] Cha J M, Kim M H, Lee S K, et al. Clin. Oncol.(R Coll Radiol), 2006, 18: 669.
[4] Tsunoda T, Eto T, Koga M, et al. Jpn. J. Surg., 1989, 19: 691.
[5] Yamaguchi K. Aust. N Z J Surg., 1992, 62: 525.
[6] Klatskin G. Am. J. Med., 1965, 38: 241.
[7] Boerma E J. Surgery, 1990, 108: 572.
[8] Bismuth H, Nakache R, Diamond T. Ann. Surg., 1992, 215: 31.
[9] Chung Y E, Kim M J, Park Y N, et al. Eur. Radiol., 2008, 18: 2182.
[10] SHEN Xue-chu(沈学础). The Techniques and Application of Modern Fourier Transform Infrared Spectroscopy(近代傅里叶变换红外光谱技术及应用). Edited by WU Jin-guang(吴瑾光主编). Beijing: Literature Press of Science and Technology(北京: 科学技术文献出版社), 1994.
[11] Lyng F M, Faolain E O, Conroy J, et al. Experimental and Molecular Pathology, 2007, 82: 121.
[12] Kretlow A, Wang Q, Kneipp J, et al. Biochim. Biophys. Acta, 2006, 1758(7): 948.
[13] LING Xiao-feng, XU Yi-zhuang, WANG Li-xin, et al(凌晓锋, 徐怡庄, 王立新, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2007, 28(3): 445.
[14] Wong P T T, Papavassiliou E D, Rigas B. Appl. Spectrosc., 1991, 45(9): 1563.
[15] LI Wei-xiu, ZHENG Quan-qing, WANG Ping, et al(李炜修, 郑全庆, 王 平, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(10): 1833.
[16] Benedetti E, Palatresi M P, Vergamini P, et al. Appl. Spectrosc., 1986, 40(1): 39.
[17] Surewicz W K, Mantsch H H. Biochim. Acta, 1988, 952: 115.
[18] Malins D C, Gunselman S J. Proc. Natl. Acad. Sci. USA, 1994, 91: 13038.
[19] Cohenford M A, Godwin T A, Cahn F, et al. Gynecol. Oncol., 1997, 66: 59.
|
| [1] |
XU Qi-lei, GUO Lu-yu, DU Kang, SHAN Bao-ming, ZHANG Fang-kun*. A Hybrid Shrinkage Strategy Based on Variable Stable Weighted for Solution Concentration Measurement in Crystallization Via ATR-FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1413-1418. |
| [2] |
KAN Yu-na1, LÜ Si-qi1, SHEN Zhe1, ZHANG Yi-meng1, WU Qin-xian1, PAN Ming-zhu1, 2*, ZHAI Sheng-cheng1, 2*. Study on Polyols Liquefaction Process of Chinese Sweet Gum (Liquidambar formosana) Fruit by FTIR Spectra With Principal Component Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1212-1217. |
| [3] |
YAN Li-dong1, ZHU Ya-ming1*, CHENG Jun-xia1, GAO Li-juan1, BAI Yong-hui2, ZHAO Xue-fei1*. Study on the Correlation Between Pyrolysis Characteristics and Molecular Structure of Lignite Thermal Extract[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 962-968. |
| [4] |
LI Zong-xiang1, 2, ZHANG Ming-qian1*, YANG Zhi-bin1, DING Cong1, LIU Yu1, HUANG Ge1. Application of FTIR and XRD in Coal Structural Analysis of Fault
Tectonic[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 657-664. |
| [5] |
CHENG Xiao-xiao1, 2, LIU Jian-guo1, XU Liang1*, XU Han-yang1, JIN Ling1, SHEN Xian-chun1, SUN Yong-feng1. Quantitative Analysis and Source of Trans-Boundary Gas Pollution in Industrial Park[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3762-3769. |
| [6] |
ZHANG Hao1, 2, HAN Wei-sheng1, CHENG Zheng-ming3, FAN Wei-wei1, LONG Hong-ming2, LIU Zi-min4, ZHANG Gui-wen5. Thermal Oxidative Aging Mechanism of Modified Steel Slag/Rubber Composites Based on SEM and FTIR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3906-3912. |
| [7] |
CHEN Jing-yi1, ZHU Nan2, ZAN Jia-nan3, XIAO Zi-kang1, ZHENG Jing1, LIU Chang1, SHEN Rui1, WANG Fang1, 3*, LIU Yun-fei3, JIANG Ling3. IR Characterizations of Ribavirin, Chloroquine Diphosphate and
Abidol Hydrochloride[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2047-2055. |
| [8] |
MA Fang1, HUANG An-min2, ZHANG Qiu-hui1*. Discrimination of Four Black Heartwoods Using FTIR Spectroscopy and
Clustering Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1915-1921. |
| [9] |
ZHANG Dian-kai1, LI Yan-hong1*, ZI Chang-yu1, ZHANG Yuan-qin1, YANG Rong1, TIAN Guo-cai2, ZHAO Wen-bo1. Molecular Structure and Molecular Simulation of Eshan Lignite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1293-1298. |
| [10] |
WANG Fang-fang1, ZHANG Xiao-dong1, 2*, PING Xiao-duo1, ZHANG Shuo1, LIU Xiao1, 2. Effect of Acidification Pretreatment on the Composition and Structure of Soluble Organic Matter in Coking Coal[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 896-903. |
| [11] |
HU Chao-shuai1, XU Yun-liang1, CHU Hong-yu1, CHENG Jun-xia1, GAO Li-juan1, ZHU Ya-ming1, 2*, ZHAO Xue-fei1, 2*. FTIR Analysis of the Correlation Between the Pyrolysis Characteristics and Molecular Structure of Ultrasonic Extraction Derived From Mid-Temperature Pitch[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 889-895. |
| [12] |
YANG Jiong1, 2, QIU Zhi-li1, 4*, SUN Bo3, GU Xian-zi5, ZHANG Yue-feng1, GAO Ming-kui3, BAI Dong-zhou1, CHEN Ming-jia1. Nondestructive Testing and Origin Traceability of Serpentine Jade From Dawenkou Culture Based on p-FTIR and p-XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 446-453. |
| [13] |
HE Xiong-fei1, 2, HUANG Wei3, TANG Gang3, ZHANG Hao3*. Mechanism Investigation of Cement-Based Permeable Crystalline Waterproof Material Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3909-3914. |
| [14] |
ZHOU Jing1,2, ZHANG Qing-qing1,2, JIANG Jin-guo2, NIE Qian2, BAI Zhong-chen1, 2*. Study on the Rapid Identification of Flavonoids in Chestnut Rose (Rosa Roxburghii Tratt) by FTIR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3045-3050. |
| [15] |
Samy M. El-Megharbel*，Moamen S. Refat. In First Time: Synthesis and Spectroscopic Interpretations of Manganese(Ⅱ), Nickel(Ⅱ) and Mercury(Ⅱ) Clidinium Bromide Drug Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3316-3320. |
|
|
|
|
