光谱学与光谱分析 |
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Investigation and Application of Fluorescence Reaction of Cerium-L-Tryptophan in Triton X-100 Microemulsion |
GONG Ai-qin1,2,ZHU Xia-shi1*,GUO Rong1,MA Xiao-qin1 |
1. Department of Chemistry, Yangzhou University, Yangzhou 225002, China 2. Yangzhou Polytechnic Institute, Yangzhou 225002, China |
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Abstract In the present article, the sensitizing effect of Triton X-100 microemulsion media (Triton X-100-M) on the determination of L-tryptophan by fluorescence spectrophotometry was studied, which was based on a strong fluorescence substance produced when L-tryptophan was oxidized by Ce4+ in acid medium. Compared with Triton X-100 micelle (Triton X-100-m), it was found that in the medium of Triton X-100-M the intensity of fluorescence was enhanced by 225 times. Various factors that influence fluorescence determination and interfering ions were examined. The mechanism of sensitizing effect of Triton X-100 microemulsion was also discussed. Under the optimum conditions, the liner range and detection limit are 0.1-1.0 μg·mL-1 and 0.09 μg·mL-1(correlation coefficient r=0.998 4), respectively. The linear equation was F=342.37+30.45c. The proposed method has been successfully applied to determine food samples.
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Received: 2006-10-29
Accepted: 2007-02-02
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Corresponding Authors:
ZHU Xia-shi
E-mail: xszhu@yzu.edu.cn
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