|
|
|
|
|
|
| Determination of Sodium Hexametaphosphate by Ratiometric Fluorescence Method Based on Formaldehyde Functionalized Polyethyleneimine/Eosin Y System |
| YANG Chuan-xiao, GONG Wei-bin, TANG Fan, SUN Xiang-ying |
| College of Materials Sciences and Engineering, Huaqiao University, Xiamen 361021, China |
|
|
|
|
Abstract Under the condition of microwave assisted, formaldehyde functionalized polyethyleneimine (FPEI) was synthesized. The maximum emission of FPEI at 470 nm was obtained when the excitation wavelength was set at 340 nm, and the blue fluorescence was easily observed under an ultraviolet lamp. Under this excitation condition, the fluorescence emission peak of the Eosin Y (EY) was located at 540 nm, and the green fluorescence was observed. In acidic medium, the EY and FPEI form a complex through electrostatic interaction. The formation of the FPEI/EY complex resulted in the quenching of EY fluorescence at 540 nm, while the fluorescence intensity of FPEI was only slightly reduced. In the presence of sodium hexametaphosphate (SHMP), competitive binding occurred between SHMP, EY and FPEI. Because the electrostatic interaction between SHMP and protonated amino groups on FPEI surface is stronger than that of EY, EY was gradually released from the PEI/EY complex, resulting in the recovery of EY fluorescence at 540 nm. The fluorescence change ratio between the fluorescence intensity of Ey at 540 nm and the fluorescence intensity of FPEI at 470 (F540/F470) has a good linear correlated with the concentration of SHMP, and a noticeable blue to green changed under an ultraviolet lamp if SHMP solution was mixed with the FPEI/EY. Based on the phenomena, a novel rapid ratiometric fluorescence method for determination of SHMP was developed. Under the optimized conditions, the linear range is 0.1~4.2 μmol·L-1, and the limit of detection (3σ) was 38 nmol·L-1. The proposed method is of high selectivity, simple and rapid, and is successfully applied to determine the concentration of SHMP in tea drink samples.
|
|
Received: 2016-03-20
Accepted: 2016-12-06
|
|
|
|
[1] YANG Hai-ran, YANG Chuan-xiao, SUN Xiang-ying(杨海冉, 杨传孝, 孙向英). Journal of Huaqiao University·Natural Science(华侨大学学报·自然科学版), 2014, 35: 413.
[2] Sun J, Yang F, Yang X R. Nanoscale, 2015, 7(39): 16372.
[3] Anema S G. International Dairy Journal, 2015, 50: 58.
[4] DAI Shao-juan, LI Lin, ZOU Sheng-Dong, et al(代绍娟，李 琳，邹圣冬，等). Food Research and Developent(食品研究与开发), 2012, 33(9): 113.
[5] Yang C X, Liu B, Sun X Y. Anal. Chim. Acta, 2012, 746: 90.
[6] Bhunia S K, Pradhan N, Jana N R. ACS Appl. Mater. Interfaces, 2014, 6(10): 7672.
[7] Ministry of Health of the People’s Republic of China. Standardization Administration of the People’s Republic of China(中华人民共和国卫生部, 中国国家标准化管理委员会). Hygienic Standards for Uses of Food Additives(食品添加剂食用卫生标准). GB2760—2007; 2007. 46.
[8] YANG Hui, LE Min, LI Yuan-fang, et al(杨 慧, 乐 敏, 李原芳, 等). SCIENTIA SINICA Chimica(中国科学: 化学)，2013, 43: 1584.
[9] Zhao H X, Luo M, Mo L X, et al. Anal. Lett., 2014, 47(16): 2740.
[10] Wen T, Li N B, Luo H Q. Anal. Chem., 2013, 85(22): 10863. |
| [1] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
| [2] |
TONG Chang-lun, XIANG Guang-hong, LIU Wei-ping. Determination of Ascorbic Acid by Indirect Fluorimetry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 598-600. |
|
|
|
|
