|
|
|
|
|
|
Rapid Detection System for HSA Based on RRS |
LI Yuan, LI Kai, ZHAO Dong-e |
School of Information and Communication Engineering, North University of China, Taiyuan 030051, China |
|
|
Abstract In the clinic, for the diagnosis of a variety of major diseases, it has great significance in terms of quick detection of concentration of human serum albumin (HSA). However, there are bulky, long test cycle, complex operations and other shortcomings for common medical detection equipment. It will have a very broad application prospects as for the design of a fast, high-precision portable detection system for HSA concentration. HSA concentration detection system was designed based on Resonance Rayleigh Scattering(RRS) spectroscopy. The mixed solution was generated with HSA and Tetraamino-phthalocyanine copper (CuTAPc) reaction, and it had a RRS enhancement effect at 475.0 nm, whereby the function of RRS intensity and HSA concentration was constructed. In the experiment, ultraviolet semiconductor lasers and 475.0 nm narrowband filter was used, the scattered light signal was collected with high gain photo-detector. Different concentrations of HSA were respectively detected with different concentrations of CuTAPc. Experimental results show that response voltage will increase significantly with the increasing of concentrations of HSA,but it is not sensitive to concentration changes for CuTAPc; It basically meet the linear change by the response voltage from scattered light and the concentration of HSA in the range from 0.1 to 1.0 μg·mL-1, and it meets the design requirements of rapid detection.
|
Received: 2016-03-10
Accepted: 2016-07-30
|
|
|
[1] Liu Y W, Liu C W, Chang H T. Talanta, 2011, 84(2): 324.
[2] Mohd, Ayaz. Journal of Fluorescence, 2011, 4(21): 1357.
[3] MA Lu, WEN Gui-qing, LIU Qing-ye(马 璐,温桂清,刘庆业). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2014, 34(9): 2481.
[4] David J Perry, David A Fitzmaurice, Steve Kitchenl. British Journal of Haematology, 2010,(150): 501.
[5] ZHANG Min, WU Li-min, ZHOU Shang(张 敏, 吴利敏, 周 尚). Journal of Instrumental Analysis(分析测试学报),2012, 6: 8.
[6] Axel Warsinke. Anal. Bioanal. Chem., 2009, 393: 1393.
[7] Ge S, Tang W, Han H, et al. J. Chromatogr. A, 2013, 1295(6): 128.
[8] Liu Jinfang, Li Nianbing, Luo Hongqun. Spectrochimica Acta, 2011, (79): 631.
[9] Ali Mohd Sajid. Journal of Colloid and Interface Science, 2010, 2(352): 436.
[10] PANG Xiang-dong, ZHENG Xiao-feng, DENG Qin-xin(庞向东, 郑晓锋, 邓钦心). Journal of Analytical Science(分析科学学报). 2011, 2: 84.
[11] Jochen S, Andra E T. Anatom. Record, 2012, 295(71): 1837.
[12] Anglstier J, Stenibegr I Z. J. Chem. Phys., 1981, 74(2): 786. |
[1] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
[2] |
REN Li-lei, PENG Yu-ling, WANG Shu-jun*, ZHANG Cheng-gen, CHEN Yu, WANG Xin-tong, MENG Xiao-ning. Fluorescence Spectroscopy for Studies on the Interaction Between Three Metalloporphyrins With Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 806-813. |
[3] |
WANG Qing-shan, WANG Dong-yang, ZHANG Xiong-jie*, TANG Bin*, WU He-xi. Research on a Decomposing Method of Energy Spectrum Overlapping Peaks Based on Gaussian Sharpening Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3245-3250. |
[4] |
HU Jing-jing, TONG Chang-lun*. Study on the Interaction Between Carbon Quantum Dots and Human Serum Albumin by Spectroscopic Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1107-1113. |
[5] |
ZHANG Jing1, GAO Xuan1, 2, JIN Liang1, WANG Hong-hui1, ZHOU Xi-ping1. Comparisons and Applications of Functional Equations for the Calculation of the Protein-Ligand Binding Constant Based on Fluorescence Spectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3494-3498. |
[6] |
YAO Dong-mei1, 2, LU Shan-shan1, WEN Gui-qing1, LIANG Ai-hui1, JIANG Zhi-liang1*. Determination of Trace Urea by Resonance Rayleigh Scattering-Energy Transfer Spectroscopy Coupled With Polystyrene Nanoprobe and Dimethylglyoxime Reaction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3590-3593. |
[7] |
GUO Qing-ying1, LIU Min1,2*, ZHAO Yan-na2, WU Yu-shu2, SUN Bin2, LIU Jie1, HAN Jun2. Spectroscopic and Cytotoxicity Study on the Influence of (-)-Epigallocatechin-3-Gallate on the Interaction of Daunorubicin with Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1821-1827. |
[8] |
ZHAO Man1, GUO Yi-xin1, HE Yu-qing1*, GUO Hong1, JIN Wei-qi1, REN Lin-mao1,2. Baseline Correction of UV Raman Spectrum Based on Improved Piecewise Linear Fitting[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1862-1868. |
[9] |
HAN Zhong-bao1, WU Yu-hang1, MI Yuan-yuan1, LIU Li-yan1,2, SU Gui-tian1, YU Zhan1,2*. Isomeric Discrimination of Oleanolic and Ursolic Acids by Human Serum Albumin: a Joint Study of Fluorescence Spectroscopy and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2190-2195. |
[10] |
XIONG Shi-peng, CHEN Jian-bo*. Characterization of the Interactions between Alpha Arbutin and Human Serum Albumin with Spectroscopic Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3489-3494. |
[11] |
WANG A-mei1, TU Zong-cai1, 2, WANG Hui1*, MA Da1. Research in Glycated Progress and Products of Human Serum Albumin by Infrared and CD Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3090-3095. |
[12] |
GONG Han-qing, CHEN Jian-bo*. The Interaction between 4-Ethyl-2-Methoxyphenol and Human Serum Albumin Studied by Spectroscopic and Molecular Docking Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1869-1873. |
[13] |
FANG Qing1, DONG Cheng-yu1, WANG Yu1, LIU Ying1,2*. The Interaction between Rolitetracycline and Human Serum Albumin Using Multi-Spectral Methods and Molecular Modeling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 990-996. |
[14] |
LIU Xing1, SHEN Peng1,2, LIANG An-wen1, YU Mei-lan1*. Study on Spectroscopy of Fullerols and Serum Albumin Interaction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3530-3536. |
[15] |
ZHANG Wan-li, ZHU Jian, LI Jian-jun, ZHAO Jun-wu*. A Fast Raman Baseline Correction Algorithm Based on Automatic Linear Fitting[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(08): 2632-2637. |
|
|
|
|