光谱学与光谱分析 |
|
|
|
|
|
Studies on Micelle Behaviors of Sophorolipid Biosurfactant by Steady-State Fluorescence Probe Method |
SONG Dan-dan1,2, LIANG Sheng-kang1,2*, WANG Jiang-tao1,2, WANG Xiu-lin1,2 |
1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China 2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China |
|
|
Abstract The critical micelle concentration (CMC) of sophorolipid was determined by steady-state fluorescence probe method in which pyrene was used as fluorescence probe. Meanwhile, the changes in the sophorolipid CMC were examined in the addition of NaCl and aliphatic alcohol, respectively. The results showed that the CMC of sophorolipid was 1.3×10-4 mol·L-1 and had a slight decrease as NaCl concentration increased. However, the CMC of sophorolipid appeared to increase in the presence of aliphatic alcohol. The aggregation numbers and the micelle size of sophorolipid solution were investigated by fluorescence quenching and laser light scattering method. The aggregation numbers were 4~8 with the concentrations of sophorolipid in the range of 4~8 CMC and the micelle mean diameter for sophorolipid with the concentration of 6 CMC was about 90 nm. These experimental results indicated that the micelle should be incompact.
|
Received: 2012-02-15
Accepted: 2012-05-10
|
|
Corresponding Authors:
LIANG Sheng-kang
E-mail: liangsk@ouc.edu.cn
|
|
[1] Ashby R D, Solaiman D K Y, Foglia T A. Biotechnology Letters, 2008, 30(6): 1093. [2] Hommel R K, Huse K. Biotechnology Letters, 1993, 15(8): 853. [3] Ratsep P, Shah V. Journal of Microbiological Methods, 2009, 78(3): 354. [4] SONG Dan-dan, LIANG Sheng-kang, WANG Jiang-tao(宋丹丹, 梁生康, 王江涛). Environmental Chemistry(环境化学), 2011, 30(8): 1474. [5] Fu S L, Wallner S R, Bowne W B, et al. Journal of Surgical Research, 2008, 148(1): 77. [6] Daverey A, Pakshirajan K. Applied Biochemistry and Biotechnology, 2009, 158(3): 663. [7] Zhang L, Somasundaran P, Singh S K, et al. Colloids and Surfaces A, 2004, 240(1-3): 75. [8] Goddard E D, Turro N J, Kuo P L. Langmuir, 1985, 1(3): 352. [9] Aguiar J, Carpena P, Molina-Bolivar J A, et al. Journal of Colloid and Interface Science, 2003, 258(1): 116. [10] YU Ya-ming, GAO Bao-jiao, JIANG Li-ding(于亚明, 高保娇, 江立鼎). Acta Chimica Sinica(化学学报), 2006, 64(18): 1859. [11] ZHANG Su-ning, ZHANG Jian, SONG Xiao-qiu, et al(章苏宁, 张 健, 宋晓秋, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2010, 27(4): 1231. [12] ZHAO Guo-xi(赵国玺). Physical Chemistry of Surfactants(表面活性剂物理化学). Beijing: Peking University Press(北京:北京大学出版社), 1991. 245,249. [13] GAN Meng-yu, LIU Yan, MA Li, et al(甘孟瑜, 刘 艳, 马 利, 等). Imaging Science and Photochemistry(影像科学与光化学), 2010, 28(2): 121. [14] Stark R E, Leff P D, Milheim S G, et al. Journal of Physical Chemistry, 1984, 88(24): 6063. [15] LIU Tian-qing, GAO Xiao-gang(刘天晴, 高小刚). Acta Physico-Chimica Sinica(物理化学学报), 2010, 26(1):66. [16] Van Bogaert I N A, Zhang J, Soetaert W. Process Biochemistry, 2011, 46(4): 821. [17] CUI Kai-long, YUAN Xing-zhong, ZENG Guang-ming, et al(崔凯龙, 袁兴中, 曾光明, 等). China Environmental Science(中国环境科学), 2011, 31(9): 1444. [18] Gadelle F, Koros W J, Schechter R S. Journal of Colloid and Interface Science, 1995, 170(1): 57.
|
[1] |
SHI Dong-po, YIN Xian-qing, ZHENG Yan-cheng, CHEN Wu, FU Jia-xin, ZOU Hua, REN Zhao-hua. HP-β-CD Reduced the Interference of the Micellization on Spectrum Quantitative Analysis of SDBS in Oilfield Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(06): 1803-1808. |
[2] |
ZHANG Jian-hua,KONG Kai-qing,HE Zheng-ling,LIU Zi-li. Determination of Critical Micelle Concentration of Alkyl Polyglucoside(APG) Nonionic Surfactant Aqueous System By Multi-Peaks Gaussian Fitting of Visible Absorption Spectra Line Shape [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(07): 1412-1415. |
|
|
|
|