光谱学与光谱分析 |
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Direct Determination of Ammonia in Plasma with Indophenol Method |
DENG Jian1, XU Jin-sheng2, FAN Hua1, YUAN Ya-li1, LIU Chuan-xiang1 |
1. College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China 2. Department of Chemistry, Hengyang Normal College, Hengyang 421008, China |
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Abstract A new method for direct determination of ammonia in plasmas with indophenol reagent is described. It is shown that using sodium citrate as anticoagulant in phosphate buffer within pH 10.5-11.2, indophenol reagent reacts sensitively with ammonia in the plasmas to form a stable blue compound with a maximum absorption at 630 nm with low interference. The apparent molor absorptivity is 2.48×104 L·mol-1·cm-1,and the linear range 0-1 500 μmol·L-1 (r=0.999 9) with a detection limit of 1.7 μmol·L-1. The relative standard deviation is 1.13%(n=11) and the recoveries are in the range of 93.6%-101.6% with an average recovery of 97.0%. The proposed method has been applied to detect ammonia in the plasmas from 43 healthy persons and 97 patients who suffered from liver diseases with satisfactory results.
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Received: 2003-09-06
Accepted: 2003-12-16
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Corresponding Authors:
DENG Jian
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Cite this article: |
DENG Jian,XU Jin-sheng,FAN Hua, et al. Direct Determination of Ammonia in Plasma with Indophenol Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 436-438.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I03/436 |
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