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Coherent Anti-Stokes Raman Scattering Imaging for Small Beads |
LI Xiang-zhao1, HOU Guo-hui1,2*, HUANG Zhi-fan1, XIAO Jun-jun2 |
1. Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518055, China
2. Department of Physics, Harbin Institute of Technology, Shenzhen 518055, China |
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Abstract This paper investigates coherent conditions for smaller anti-Stokes Raman scattering imaging sample size than the system point spread function size and the reasons for depth around coherent anti-Stokes Raman scattering images using experimental design and analysis models. The axial transmission dynamic displacement light method, which subtracts axial light from the coherent volume element, is introduced to model and analyse lateral and axial dimensions for spherical or cylindrical samples with a smaller diameter than the system point spread function. The Gouy phase shift effect was approximately zero for small sample size and large refractive index. The main reason was the interaction between sample refractive index and the surrounding environment, and the system coherent tomographic volume element effective length. The obtained results apply only to CARS image analysis, where the sample size is smaller than the system point spread function, but it is also the first paper that clarifies the underlying reason for depth around CARS images using design experiments and quantitative model analysis. Successful nano-imaging analysis using axial transmission dynamic displacement light verified that the nano-action mechanism is similar to coherent action length, effective action length and its travel path.
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Received: 2020-09-08
Accepted: 2021-01-19
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Corresponding Authors:
HOU Guo-hui
E-mail: houguohui_2008@163.com
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