| 光谱学与光谱分析 |
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| Determination of Trace Free Copper and Zinc in Serum Samples by Graphite Furnace Atomic Absorption Spectrometry after Two-Step Precipitation of Protein |
| ZHANG Yuan,LIN Zhe-xuan,LI Wei-qiu,ZHANG Qiao-xin,LI Hui,LUO Hong-jun,LUO Wen-hong* |
| Central Laboratories, Shantou University Medical College, Shantou 515041, China |
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Abstract A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1∶2. The mixture was subsequently denatured at 70 ℃ and centrifuged to precipitate proteins. The determination.limit of copper (3σ) was 1.2 μg·L-1 and the recovery was 92.3%-104%, and the determination. Limit of zinc(3σ) was 0.098 μg·L-1 and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people.
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Received: 2008-05-06
Accepted: 2008-08-08
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Corresponding Authors:
LUO Wen-hong
E-mail: whluo@stu.edu.cn
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