Study of Raman Spectra of Single Carcinoma of Nasopharynx Cell
YAO Hui-lu1, ZHU Miao2, WANG Gui-wen1, PENG Li-xin1, HE Bi-juan3, HE Min2,LI Yong-qing4
1.Laboratory for Biophysics, Guangxi Academy of Sciences, Nanning 530003，China 2. Laboratory Center of Guangxi Medical University, Nanning 530021, China 3. Guangxi Academy of Sciences, Nanning 530003，China 4. Department of Physics, East Carolina University, Greenville, North Carolina 27858-4353, USA
Abstract：The Raman spectra from carcinoma of nasopharynx cell lines (CNE2) and normal airway epithelial cell lines(HBE) were investigated using a laser tweezers Raman spectroscopy (LTRS). The Raman scattering measurements were obtained from three different places in every single cell.Visual inspection of the spectra shows that the differences observed in spectra of the cancer cells and normal cells are obvious. The peak ratio I1304/I1336 is 1.05 for the normal cell and 1.22 for the cancer cell. Using a combination of principal component analysis(PCA) and discriminant function analysis(DFA), the authors are able to predict cancer cells, and normal cells and the DFA is better for single Raman spectrum. The sampling locations did not seriously affect the result of PCA and DFA. PCA and DFA also show that the uniformity of normal cells is better than that of cancer cells. The results indicate that the Raman spectra may offer the experimental basis for colorectal cancer diagnosis.
Key words：Raman spectroscopy;Carcinoma of nasopharynx;Laser tweezer;Principal component analysis;Discriminant function analysis
姚辉璐1,朱淼2,王桂文1,彭立新1,何碧娟3,何敏2,黎永青4 . 单个鼻咽癌细胞的拉曼光谱分析的研究[J]. 光谱学与光谱分析, 2007, 27(09): 1761-1764.
YAO Hui-lu1, ZHU Miao2, WANG Gui-wen1, PENG Li-xin1, HE Bi-juan3, HE Min2,LI Yong-qing4 . Study of Raman Spectra of Single Carcinoma of Nasopharynx Cell. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1761-1764.
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