Study on the Change of the Content of Aromatic Amino Acid Residues in the Process of Malignant Transformation by Synchronous Fluorescence Spectrometry
ZHANG Yuan1, LIN Zhe-xuan1, HAN Ming2*
1. Bio-analytical Laboratory, Shantou University Medical College, Shantou 515041, China
2. Emergency Department of the Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, China
Abstract：Synchronous fluorescence spectra was applied to assess tyrosine (Tyr) and tryptophane (Trp) residues in the plasma of patients suffering hepatocellular carcinoma, tumor-bearing mice, and cultured cells (HepG2 and HL-7702). The synchronous fluorescence peaks of Tyr and Trp residues were at 318 and 350 nm when Δλ=20 nm and Δλ=80 nm respectively, and did not shift regardless of the sample variations. The results showed that the fluorescent intensities of Tyr and Trp residues increased significantly in the plasma proteins of patients suffering hepatocellular carcinoma. There was a correlation between the increased fluorescent intensities of murine plasma Tyr or Trp residues and the increasing of time of tumor-bearing, indicating that the alterations of Tyr and Trp residues might be associated with tumor development. On the contrary, the fluorescent intensities of Tyr and Trp residues in tumor tissue or HepG2 cells decreased along with the increasing of time of tumor-bearing or culturing. Further experiments showed that a potential anti-tumor medicine-matrine which exerted an anti-tumor activity could enhance the fluorescence intensities of Tyr and Trp residues. These results demonstrated that synchronous fluorescence spectra could be used to determine Tyr and Trp residues in proteins. By this method, we found that Tyr and Trp residues increased in the plasma proteins of tumor bearing patients and mice which might be correlated with tumor development. On the contrary, Tyr and Trp residues in proteins of tumor tissue or cancer cells decreased. It remains unclear whether these changes acted as a biomarker for protein metabolism imbalance or they contributed to the tumor evolution in malignant diseases, which is worthy of further investigation.
张 源，林哲绚，韩 溟. 同步荧光光谱法研究肿瘤芳香族氨基酸残基含量的变化[J]. 光谱学与光谱分析, 2017, 37(09): 2822-2825.
ZHANG Yuan, LIN Zhe-xuan, HAN Ming. Study on the Change of the Content of Aromatic Amino Acid Residues in the Process of Malignant Transformation by Synchronous Fluorescence Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(09): 2822-2825.
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