The Advancement of Attenuated Total Reflection Fourier Transform Infrared Technology in Clinical Application
ZHANG Xiao-qing1, SUN Xiao-liang2, PAN Qing-hua3, ZHANG Yuan-fu4, XU Zhi5*, GUO Xiang-yang1*
1. Anesthesia Department, Peking University Third Hospital, Beijing 100191, China 2. General Surgery, China-Japan Friendship Hospital, Beijing 100029, China 3. Hangzhou Normal University, Hangzhou 311121, China 4. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 5. General Department, Peking University Third Hospital, Beijing 100191, China
摘要： 系统回顾了衰减全反射傅里叶变换红外光谱(attenuated total reflection Fourier transform infrared, ATR-FTIR)技术在临床医学领域中的应用研究进展。ATR-FTIR光谱技术具有实时、简单、无创扫描生物组织样品的优势，通过结合多模式识别统计学方法并对照临床及病理学诊断结果，可对生物组织样本进行判别分析。目前，对甲状腺、乳腺，以及肺组织等新鲜离体组织的光谱学检测中，ATR-FTIR技术可达到较高的判别敏感性、准确性与特异性;尤其在对甲状腺和乳腺疾病患者的前哨淋巴结活检的研究中，应用ATR-FTIR技术，可对良、恶性组织进行敏感性、准确性与特异性均较高的鉴别研究，对辅助临床诊断具有潜在的重要价值。此外，利用ATR-FTIR光谱技术对生物体液进行光谱分析并进一步诊断疾病的研究也在逐步开展。由于血清中所含成分的改变对于提示疾病状况具有重要价值，因而血清ATR-FTIR光谱学诊断方法成为研究热点，已有应用ATR-FTIR技术对脑胶质细胞瘤、心肌梗死、肾衰、阿尔茨海默病、卵巢癌与子宫内膜癌等多种疾病进行分析的研究报道。目前已可通过ATR-FTIR技术对卵巢癌进行疾病分期的判别诊断，该技术有望成为卵巢癌筛查的重要方法。由于血清ATR-FTIR光谱学技术具有检测快速、判别准确、性价比高等优势，有望成为今后生物医学发展的重要方向之一。
关键词：衰减全反射;傅里叶变换红外光谱;血清;恶性肿瘤
Abstract：The authors systemically reviewed the fast development of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and its clinical application in the past decades. The advantages of this objective technique include real time scanning, easy manipulation and no harm to the subjects examined. Combined with pattern recognition methodology and further confirmation with the clinical and pathological diagnosis, the goal of fast differentiation of malignancy from benign lesions could be achieved. ATR-FTIR spectroscopy technique has shown high differential capacity for benign and malignant tissues such as thyroid, breast and pulmonary diseases. ATR-FTIR spectroscopy has being applied in investigating the differential value (the sensitivity, specificity, and accuracy) of metastatic lymph nodes in thyroid and breast cancer with encouraging results. ATR-FTIR technique would become a promising tool in tissue diagnosis intra-operatively. ATR-FTIR spectroscopy has also been widely applied in detecting bio-fluid to differentiate diseases. The serum ATR-FTIR spectroscopy has the ability of reflecting disease-related information in a fingerprint manner with little amount of blood. Several published articles have covered diseases such as glioma, chest pain, prostate cancer, renal failure, Alzheimer’s disease, and ovarian cancer. The results of these researches have proved the efficacious discriminate value of this method. As ATR-FTIR spectroscopy has the potential of fast analysis, accurate diagnosis, and low cost-effective value. It would become one of the most important assisting diagnosis tools in future. Follow-up study should focus on enhancing sample quality and enlarging sample size to have further prospective clinical application.
Key words：Attenuated total reflection;Fourier transform infrared spectroscopy;Serum;Malignancy
[1] Glassford S E, Byrne B, Kazarian S G. Biochim. Biophys. Acta，2013, 1834(12); 2849. [2] Zhang X, Thiéfin G, Gobinet C, et al. Transl. Res.，2013, 162(5)：279. [3] Dorling K M, Baker M J. Trends Biotechnol.，2013, 31(6)：327. [4] ZHOU Xiao-si, XU Zhi, LING Xiao-feng, et al(周孝思，徐 智，凌晓峰，等). Chinese Journal of Oncology(中华肿瘤杂志)，2006, 28(7)：512. [5] Tian P, Zhang W, Zhao H, et al. Int. J. Clin. Exp. Med., 2015, 8(1)：972. [6] Tian P, Zhang W, Zhao H, et al. Br. J. Surg., 2015, 102(11): 1372. [7] Zhang X, Xu Y, Zhang Y, et al. J. Surg. Res., 2011, 171(2): 650. [8] Liu Y, Xu Y, Liu Y, et al. Br. J. Surg., 2011, 98(3): 380. [9] Sun X, Xu Y, Wu J, et al. J. Surg. Res., 2013, 179(1): 33. [10] Gajjar K, Heppenstall L D, Pang W, et al. Anal. Methods, 2012, 6(5): 89. [11] Lima C A, Goulart V P, Crrea L, et al. Int. J. Mol. Sci., 2015, 16(4): 6621. [12] Theophilou G, Lima K M, Martin-Hirsch P L, et al. Analyst，2015, 19. [13] Hands J R, Abel P, Ashton K, et al. Anal. Bioanal. Chem., 2013, 405(23): 7347. [14] Hands J R, Dorling K M, Abel P, et al. J Biophotonics，2014, 7(3-4)：189. [15] Petrich W, Lewandrowski K B, Muhlestein J B, et al. Analyst，2009, 134(6)：1092. [16] Ollesch J, Drees S L, Heise H, et al. Analyst，2013, 138(14)：4092. [17] Gajjar K, Trevisan J, Owens G, et al. Analyst，2013, 138(14)：3917. [18] Lima K M, Gajjar K B，Martin-Hirsch P, et al. Biotechnol. Prog.，2015, 31(3)：832. [19] Khanmohammadi M, Ghasemi K, Garmarudi A B, et al. Spectrochim Acta A Mol. Biomol. Spectrosc., 2014, 136: 1782. [20] Sitole L, Steffens F, Krüger T P, et al. OMICS，2014, 18(8)：513. [21] Rabia Sarroukh, Erik Goormaghtigh, Jean-Marie Ruysschaert, et al. Biochimica et Biophysica Acta，2013, 1828(10)：232. [22] Mitchell A L, Gajjar K B, Theophilou G, et al. J. Biophotonics, 2014, 7(3-4): 153. [23] Caryn Hughes, Michael Brown, Graeme Clemens, et al. Journal of Biophotonics, 2014, 7(4)：180.
