Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses
CHAI A-li1, WANG Yi-kai2, ZHU Fa-di1, SHI Yan-xia1, XIE Xue-wen1, LI Bao-ju1*
1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China
Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses
CHAI A-li1, WANG Yi-kai2, ZHU Fa-di1, SHI Yan-xia1, XIE Xue-wen1, LI Bao-ju1*
1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China
摘要： Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic examination and can be influenced by the interpretation of the micro-morphological characters observed. The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR) spectroscopy. In this study, FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fungi of horticultural plants. Mixtures of mycelia and spores from 27 fungal strains belonging to nine different families were collected from liquid PD or solid PDA media cultures and subjected to FTIR-ATR spectroscopy measurements. The FTIR-ATR spectra ranging from 4 000 to 400 cm-1 were obtained. To classify the FTIR-ATR spectra, cluster analysis was compared with canonical vitiate analysis (CVA) in the spectral regions of 3 050～2 800 and 1 800～900 cm-1. Results showed that the identification accuracies achieved 97.53% and 99.18% for the cluster analysis and CVA analysis, respectively, demonstrating the high potential of this technique for fungal strain identification.
Abstract：Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic examination and can be influenced by the interpretation of the micro-morphological characters observed. The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR) spectroscopy. In this study, FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fungi of horticultural plants. Mixtures of mycelia and spores from 27 fungal strains belonging to nine different families were collected from liquid PD or solid PDA media cultures and subjected to FTIR-ATR spectroscopy measurements. The FTIR-ATR spectra ranging from 4 000 to 400 cm-1 were obtained. To classify the FTIR-ATR spectra, cluster analysis was compared with canonical vitiate analysis (CVA) in the spectral regions of 3 050～2 800 and 1 800～900 cm-1. Results showed that the identification accuracies achieved 97.53% and 99.18% for the cluster analysis and CVA analysis, respectively, demonstrating the high potential of this technique for fungal strain identification.
CHAI A-li1, WANG Yi-kai2, ZHU Fa-di1, SHI Yan-xia1, XIE Xue-wen1, LI Bao-ju1* . Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses[J]. 光谱学与光谱分析, 2016, 36(11): 3764-3771.
CHAI A-li1, WANG Yi-kai2, ZHU Fa-di1, SHI Yan-xia1, XIE Xue-wen1, LI Bao-ju1* . Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(11): 3764-3771.
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