Micro-Raman Spectra for Gastritis and Gastric Ulcer Tissues
WANG Hui-min1, ZHANG Jin-yan1, GUO Jian-yu1, CAI Wei-ying1, SUN Zhen-rong1*, WANG Zu-geng1, FANG Min2, SUN Man-ping2, MA Shu-ying2
1. Key Laboratory of Optical and Magnetic Resonance Spectroscopy (East China Normal University), Ministry of Education, and Department of Physics, East China Normal University, Shanghai 200062, China 2. Yue-yang Hospital of Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
Abstract：Micro-Raman spectroscopy was employed to identify gastritis tissues and gastric ulcer tissues. The primary spectral differences between the two types of samples include, for gastric ulcer tissues, (1) the intensity of the peak at 781 cm-1 ascribed to cytosine decreases, while the peaks ascribed to adenine and thymine respectively at 793 and 823cm-1 become stronger; (2) the bands of amide Ⅰ and amide Ⅲ at 1654 and 1320-1270cm-1 respectively, characteristic of α-helix structural protein, lose their intensities, and the tryptophan band at 1332cm-1 and phenylalanine band at 1003cm-1 reduced significantly, while the tryptophan marker at 1554cm-1 up shiftes to 1556cm-1 with increasing intensity; (3) a blue shift of 1073cm-1 line, the characteristic Raman band of lipid, and a reduction in the ratio of 1303 cm-1 assigned to in-phase CH2 twisting motion to 1268cm-1 from CH in-plane deformation were observed; (4)statistic analysis shows that the ratio of Raman intensities at bands 1449 cm-1 originating from CH2 group to 1660cm-1 from amideⅠ provides a promising standard to distinguish the two tissues.
王慧敏1,张金彦1,郭建宇1,蔡炜颖1,孙真荣1*,王祖赓1,房敏2,孙曼萍2,马淑颖2 . 胃炎和胃溃疡组织细胞的显微拉曼光谱研究[J]. 光谱学与光谱分析, 2007, 27(10): 2038-2041.
WANG Hui-min1, ZHANG Jin-yan1, GUO Jian-yu1, CAI Wei-ying1, SUN Zhen-rong1*, WANG Zu-geng1, FANG Min2, SUN Man-ping2, MA Shu-ying2. Micro-Raman Spectra for Gastritis and Gastric Ulcer Tissues. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2038-2041.
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