Determination of Lead and Cadmium in Calendula Officinalis Seedlings for Phytoremediation of Multi-Contaminated Loess by Using Flame Atomic Absorption Spectrometry with Wet Digestion
FAN Chun-hui1, DU Bo1, ZHANG Ying-chao2, GAO Ya-lin1, CHANG Min1
1. School of Environmental Science & Engineering, Shaanxi University of Science & Technology, Xi’an 710021, China 2. School of Environment, Tsinghua University, Beijing 100084, China
Abstract：Atomic absorption spectrometry (AAS) is widely used in the analysis and detection of heavy metals. The optimal operation conditions on metals detection are important for the stabilization and repeatability of scientific research, which affects the expected investigation objectives greatly. Thus, it becomes a key issue to establish appropriate detection methods in scientific works. Calendula officinalis was used for lead/cadmium remediation in contaminated loess, and the flame atomic absorption spectrometry (FAAS) with wet digestion was applied to analyze the contents of lead/cadmium in Calendula officinalis seedlings. The investigation data was further studied to reveal the bioaccumulation efficiency of lead/cadmium in Calendula officinalis seedlings. The results showed: the limits of detection are 0.104 and 0.007 mg·L-1 for lead and cadmium; the recovery rates of lead and cadmium are from 94.33% to 110.78% and 97.73% to 107.50% respectively. The relative standard deviations (RSD) are between 4.11% and 4.75% for lead while between 1.11% and 2.77% for cadmium，and It’s been proved that the method is accurate and reliable. The low accumulation efficiency of lead with Calendula officinalis seedlings might be related to the electronegativity of lead while the growth period of calendula officinalis seedlings and environmental factors. The accumulation content of cadmium is 104.85 mg·kg-1 with cadmium concentration of 50 mg·kg-1. The co-existing of lead in loess is positive for cadmium accumulation by Calendula officinalis seedlings, and the synergistic effect might work in the process. The established detection method is effective for quantitative analysis of lead and cadmium in calendula officinalis seedlings and significant for future research.
范春辉1，杜 波1，张颖超2，高雅琳1，常 敏1 . 湿法消解火焰原子吸收法测定黄土复合污染修复植物金盏菊幼苗中的铅和镉 [J]. 光谱学与光谱分析, 2016, 36(08): 2625-2628.
FAN Chun-hui1, DU Bo1, ZHANG Ying-chao2, GAO Ya-lin1, CHANG Min1 . Determination of Lead and Cadmium in Calendula Officinalis Seedlings for Phytoremediation of Multi-Contaminated Loess by Using Flame Atomic Absorption Spectrometry with Wet Digestion. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(08): 2625-2628.
[1] Chaney R L, Malik M, Li Y M, et al. Current Opinions in Biotechnology, 1997, 8: 279. [2] Loescher C M, Morton D W, Razic S, et al. Journal of Pharmaceutical and Biomedical Analysis, 2014, 98: 52. [3] Vigil M, Marey-Pérez M F, Huerta G M, et al. Science of the Total Environment, 2015, 505: 844. [4] Roccotiello E, Serrano H C, Mariotti M G, et al. Chemosphere, 2015, 119: 1372. [5] BAO Shi-dan(鲍士旦). Agrochemistry Analysis(土壤农化分析), 3rd Edition(3版). Beijing: China Agriculture Press(北京: 中国农业出版社), 2005. 373. [6] MacDougall D, Crummett W B. Analytical Chemistry, 1980, 52: 2242. [7] Mani D, Kumar C, Patel N K. Ecotoxicology and Environmental Safety, 2015, 111: 86. [8] Bradl H B. Journal of Colloid and Interface Science, 2004, 277: 1. [9] Ali H, Khan E, Sajad M A. Chemosphere, 2013, 91: 869. [10] Liu J N, Zhou Q X, Sun T, et al. Journal of Hazardous Materials, 2008, 151: 261.
