Study on the Genetic Relationship of Panax Notoginseng and Its Wild Relatives Based on Fourier Translation Infrared Spectroscopy
LI Yun1, 2, WANG Yuan-zhong2, YANG Wei-ze2, YANG Shao-bing2, ZHANG Jin-yu1, 2*, XU Fu-rong1*
1. College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China 2. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
Abstract:Wild relatives play a very important role in enriching germplasm resources and improving the quality and yield of cultivated species. In this paper, the genetic relationship between Panax notoginseng and its wild relatives has been investigated by using Fourier transform infrared (FTIR) spectroscopy in order to provide theoretical bases in the variety improvement of P. notoginseng as well as the development and utilization of germplasm resources. The FTIR spectra of P. notoginseng and its wild relatives (P. japonicus var. major, P. stipuleanatus, P. vietnamensis, P. japonicus var. bipinnatifidus) as well as Panax notoginsenosides were collected. The original infrared spectra of P. notoginseng and its wild relatives were pretreated by automatic baseline correction, smoothing, ordinate normalization and second derivative. The genetic relationship between P. notoginseng and its wild relatives has been studied together with the aid of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). By comparing the infrared spectra of P. notoginseng with that of panax notoginsenosides, some common peaks such as 3 400, 2 930, 1 635, 1 385, 1 075 and 927 cm-1 has been found. It showed that the peak heights of P. notoginseng samples may relate with the content of panax notoginsenosides. The original infrared spectra of P. notoginseng are similar to its wild relatives and the absorption peaks of the functional groups of C—H, CO, O—H, C—N and C—O were presented. There were some differences in the fingerprint region (1 800~500 cm-1) of the second derivative spectra of these five species samples. The characteristic absorption peaks such as 1 385 and 784 cm-1 has an obviously differentiation. Then the fingerprint region of second derivative spectra is subjected to be analyzed by PCA and PLS-DA. By comparing the 3D score plots of these two methods, the classification result of PLS-DA is significantly better than PCA. In addition, the result of HCA which based on the six principal components of PLS-DA has shown that P. japonicus var. major and P. vienamensis have close relationship with P. notoginseng while P. stipuleanatus and P. japonicus var. bipinnatifidus are far from P. notoginseng. The use of Fourier transform infrared spectroscopy combined with chemometrics methods could effectively investigate the genetic relationship between P. notoginseng and its wild relatives. Furthermore, it could provide reference for the research of medicinal plants.
李 运1, 2,王元忠2,杨维泽2,杨绍兵2,张金渝1, 2*,徐福荣1* . 红外光谱对三七及其野生近缘种亲缘关系研究 [J]. 光谱学与光谱分析, 2016, 36(08): 2420-2424.
LI Yun1, 2, WANG Yuan-zhong2, YANG Wei-ze2, YANG Shao-bing2, ZHANG Jin-yu1, 2*, XU Fu-rong1* . Study on the Genetic Relationship of Panax Notoginseng and Its Wild Relatives Based on Fourier Translation Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(08): 2420-2424.
[1] Ng T B. Journal of Pharmacy and Pharmacology, 2006, 58(8): 1007. [2] Zhou S L, Xi G M, Li Z Y, et al. Journal of Integrative Plant Biology, 2005, 47(1): 107. [3] YANG Chong-ren, CHEN Ke-ke, WANG Dong, et al(杨崇仁, 陈可可, 王 东, 等). The 9th National Academic Symposium on Medicinal Plants and Plant Medicine(第九届全国药用植物及植物药学术研讨会论文集), 2010: 26. [4] ZHANG Zi-long, WANG Wen-quan, YANG Jian-zhong, et al(张子龙, 王文全, 杨建忠, 等). Soils(土壤), 2010, 42(6): 1009. [5] Maxted N, Ford-Lloyd B V, Jury S, et al. Conservation, 2006, 15(8): 2673. [6] LU Bao-rong(卢宝荣). Chinese Science Bulletin(科学通报), 2014, (6): 479. [7] Brar D S, Khush G S. Plant Molecular Biology, 1997, 35(1-2): 35. [8] SUN Yu-qin, CHEN Zhong-jian, ZHOU Shi-liang, et al(孙玉琴, 陈中坚, 周世良, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2009, 34(20): 2567. [9] Amirabadizadeh H, Jafari A, Mahmoodzadeh H. Nordic Journal of Botany, 2015, 33(2): 159. [10] Martins S, Simes F, Matos J, et al. Plant Systematics and Evolution, 2014, 300(5): 1035. [11] Sharma S K, Dawson I K, Waugh R. Theoretical and Applied Genetics, 1995, 91(4): 647. [12] Cheng C, Liu J, Zhang C, et al. Applied Spectroscopy Reviews, 2010, 45(2): 148. [13] Fan Q, Chen C, Lin Y, et al. Journal of Molecular Structure, 2013, 1051: 66. [14] Kwon Y K, Ahn M S, Park J S, et al. Journal of Ginseng Research, 2014, 38(1): 52. [15] Yap K Y L, Chan S Y, Lim C S. Journal of Biomedical Science, 2007, 14(2): 265. [16] Demir P, Onde S, Severcan F. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, 135: 757. [17] Mariey L, Signolle J P, Amiel C, et al. Vibrational Spectroscopy, 2001, 26(2): 151. [18] ZHANG Zhi-xin, ZHANG Shi-xiu(张志信, 张仕秀). Lishizhen Medicine and Materia Medica Research(时珍国医国药), 2012, 23(12): 3126. [19] SUN Su-qin(孙素琴). Analysis of Traditional Chinese Medicine by Infrared Spectroscopy(中药红外光谱分析与鉴定). Beijing: Chemical Industry Press(北京:化学工业出版社), 2010. [20] Li D, Jin Z, Zhou Q, et al. Journal of Molecular Structure, 2010, 974(1): 68. [21] Abdi H, Williams L J. Wiley Interdisciplinary Reviews: Computational Statistics, 2010, 2(4): 433. [22] Mellado-Mojica E, López M G. Food Chemistry, 2015, 167: 349. [23] Luna A S, da Silva A P, Pinho J S A, et al. Food Research International, 2015, 67: 206. [24] Ciosek P, Brzózka Z, Wróblewski W, et al. Talanta, 2005, 67(3): 590. [25] Pérez-Enciso M, Tenenhaus M. Human Genetics, 2003, 112(5-6): 581. [26] Demir P, Onde S, Severcan F. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, 135: 757. [27] Qiu L, Wang Z, Liu P, et al. Spectroscopy Letters, 2015, 48(2): 120. [28] ZHANG Jin-yu, YANG Wei-ze,CUI Xiu-ming, et al(张金渝, 杨维泽, 崔秀明, 等). Journal of Plant Genetic Resources(植物遗传资源学报), 2011, 12(2): 249.