Abstract:The in situ biological process relays on a high performance attenuated total reflection (ATR) probe while ATR technique is a powerful tool for inline analysis. The basic principle and characteristic of the ATR technique was analyzed in this paper. A low cost and high throughout ATR probe was designed which can be inserted into the biological reactor directly with nondestructive and zero delay monitoring. It shows that the online ATR probe has a wide spectrum range, and the collected spectra has a high signal-to-noise ratio, which can be used in in-line quantitative analysis for biological process.
石 磊1,刘 佳2,郜 武1,张倩暄1,王 巍1. 一种在线监测的ATR探头的设计[J]. 光谱学与光谱分析, 2016, 36(06): 1954-1958.
SHI Lei1,LIU Jia2,GAO Wu1,ZHANG Qian-xuan1,WANG Wei1. The Design of an ATR Probe for Online Monitoring of Biological Process. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(06): 1954-1958.
[1] Gunta Mazarevica, Josef Diewok, Josefa R Baena,et al. Applied Spectroscopy, 2004, 58(7): 804. [2] Jakob J Muller, Markus Neumann, Paul Scholl,et al. Analytical Chemistry, 2010, 82(14): 6008. [3] Cathryn L McFearin, Jagadis Sankaranarayanan, Adah Almutairi. Analytical Chemistry,2011, 83(10): 3943. [4] Daniel Lumpi, Christoph Wagner, Matthias Schopf, et al. Chemical Communications,2012, 48(18): 2451. [5] Philippa Alice Hayes, Signe Vahur, Ivo Leito. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014, 133(10): 207. [6] Daniel Lumpi,Christian Braunshier. Effective Reaction Monitoring of Intermediates by ATR-IR Spectroscopy Utilizing Fibre Optic Probes, Infrared Spectroscopy-Materials Science, Engineering and Technology, Theophanides Theophile (Ed.),2012. [7] Riley M R, Rhiel M, Arnold M A,et al. Biotechnol. Bioeng., 1997, 55(1): 11. [8] Cozzolino D, Curtin C. Food Control,2012, 26(2): 241. [9] Francis M Mirabella. Internal Reflection Spectroscopy: Theory and Applications: CRC Press,1992. [10] Milan Milosevic. Internal Reflection and ATR Spectroscopy: John Wiley & Sons,2012. [11] John Dahlbacka, Jan Weegar, Niklas Weymarn,et al. Biotechnol Letter, 2012, 34(6): 1009. [12] James A Harrington. Hollow Waveguides Fiber and Integrated Optics, 2000, 19(3): 211. [13] Clemens B Minnich, Pascal Buskens, H Christian Steffens, et al. Organic Process Research & Development 2007, 11(1): 94.