Abstract：Chitin is an important structural polysaccharide of fungal cell wall. In this paper, aerial hyphae of Colletotrichum camelliae Massee was first studied by confocal Raman microscopy in vivo. Firstly, the optimal experimental parameters of hyphae for collecting the Raman spectra were determined, and the typical Raman spectra of hyphae, chitin standard and background were acquired. By comparing analysis, characteristic peaks of chitin were found in hyphae. Then, a region of interesting on hyphae was selected for Raman scanning. Through principal component analysis, the Raman signal of hyphae and background in the scanning area can be separated clearly. Combined with loading weight plot, two main characteristic peaks of hyphae were obtained, 1 622 cm-1was belong to chitin and 1 368 cm-1 was assigned to pectic polysaccharide. Finally, two and three dimension chemical images of fungal hyphae were realized based on Raman fingerprint spectra of chitin in a nondestructive way.
李晓丽1，罗榴彬1，周斌雄1，胡小倩2，孙婵骏1，何 勇1* . 基于共聚焦显微拉曼的真菌菌丝中几丁质的原位检测研究 [J]. 光谱学与光谱分析, 2016, 36(01): 119-124.
LI Xiao-li1, LUO Liu-bin1, ZHOU Bin-xiong1, HU Xiao-qian2, SUN Chan-jun1, HE Yong1* . In Vivo Study of Chitin in Fungal Hyphae Based on Confocal Raman Microscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(01): 119-124.
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