Application of A New Spectrophotometric Method Manipulating Ratio Spectra for Determination of Bambuterol Hydrochloride in the Presence of Its Degradation Product Terbutaline
Nesrine T. Lamie
Cairo University, Faculty of Pharmacy, Department of Analytical Chemistry, Kasr El-Aini Street, ET 11562, Cairo, Egypt
Application of A New Spectrophotometric Method Manipulating Ratio Spectra for Determination of Bambuterol Hydrochloride in the Presence of Its Degradation Product Terbutaline
Nesrine T. Lamie
Cairo University, Faculty of Pharmacy, Department of Analytical Chemistry, Kasr El-Aini Street, ET 11562, Cairo, Egypt
摘要： A simple, specific, accurate and precise spectrophotometric stability indicating method is developed for determination of bambuterol hydrochloride (BH) in the presence of its degradation product terbutaline (TERB) and in pharmaceutical formulations. A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 245 and 260 nm of ratio spectra. The calibration curves are linear over the concentration range of 0.1～1 mg·mL-1 for BH and 0.1～0.7 mg·mL-1 for TERB with mean percentage recovery of 100.56±0.751 and 99.88±1.183, respectively. The selectivity of the proposed method is checked using laboratory prepared mixtures. The proposed method has been successfully applied to the analysis of BH in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with pharmacopeial method.
Abstract：A simple, specific, accurate and precise spectrophotometric stability indicating method is developed for determination of bambuterol hydrochloride (BH) in the presence of its degradation product terbutaline (TERB) and in pharmaceutical formulations. A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 245 and 260 nm of ratio spectra. The calibration curves are linear over the concentration range of 0.1～1 mg·mL-1 for BH and 0.1～0.7 mg·mL-1 for TERB with mean percentage recovery of 100.56±0.751 and 99.88±1.183, respectively. The selectivity of the proposed method is checked using laboratory prepared mixtures. The proposed method has been successfully applied to the analysis of BH in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with pharmacopeial method.
通讯作者:
Nesrine T. Lamie
E-mail: nesrinelamie@hotmail.com
引用本文:
Nesrine T. Lamie . Application of A New Spectrophotometric Method Manipulating Ratio Spectra for Determination of Bambuterol Hydrochloride in the Presence of Its Degradation Product Terbutaline[J]. 光谱学与光谱分析, 2015, 35(01): 151-156.
Nesrine T. Lamie . Application of A New Spectrophotometric Method Manipulating Ratio Spectra for Determination of Bambuterol Hydrochloride in the Presence of Its Degradation Product Terbutaline. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(01): 151-156.
[1] Sweetman S C. Martindale: The Complete Drug Reference. 33rd ed. London: Pharmaceutical Press, 2002. 761. [2] O’Neil M J, Smith A, Heckelman P E, et al. Merck Index. 13th ed. John Wiley and Sons, 2001. 166. [3] The British Pharmacopoeia, Her Majesty Stationary Office, London, UK: 2007. 211. [4] Zhang Y. J. Pharm. Clin. Res., 2001, 9: 13. [5] Bartolincic A, Druskovic V, Sporec A, et al. J. Pharm. Biomed. Anal., 2005, 36: 1003. [6] Wannerberg O, Persson B. J. Chromatogr. A, 1988, (435): 199. [7] Harris R, Hodgkinson P, Larsson T, et al. J. Pharm. Biomed. Anal., 2005, (38): 858. [8] Di Corcia D, Morra V, Pazzi M, et al. Biomed. Chromatogr., 2010, 24(4): 358. [9] Amr M Badawy, Nadia M Mostafa, Abd El-Aziz B Abd El-Aleem, et al. Int. J. Comprehensive Pharm. IJCP, 2011, 6(10). [10] Liu L, Li K X, Shi A X, et al. Chinese Journal of Pharmaceutical Analysis, 2001, 21(5): 316. [11] Nadia M Mostafa, Amr M Badawey, Abd El-Aziz B Abd El-Aleem Nesrine T Lamie. Journal of Applied Pharmaceutical Science，2011，1(5)：191. [12] Shi A X, Li K X, Cao G Y, et al. Chinese Journal of Pharmaceutical Analysis, 2001, 21(4): 290. [13] Lotfy H M, Hegazy M A. Spectrochim. Acta A，2012，96：259. [14] Spiegel M R，Stephens L J. Schaum Outline of Theory and Problems of Statistics, Schaum Outline Series, New York, 1999.
