1. 云南民族大学民族药资源化学国家民委-教育部重点实验室，云南 昆明 650500 2. Center for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, VIC 3216, Australia
Studies on the Interaction between Malanin and Metal Ions by Fluorescence Spectra Methods
YUAN Yan1, ZHANG Wei1, DAI Jian-hui1, LIU Xiao-fang1, WANG Hong-bin1, Colin J Barrow2, YANG Wen-rong2
1. Key Laboratory of Chemistry in Ethnic Medicinal Resources，State Ethnic Affairs Commission and Ministry of Education，Yunnan Minzu University, Kunming 650500, China 2. Center for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, VIC 3216, Australia
Abstract：A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37×10-4 and 2.66×10-4 mol·L-1, respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37×10-4 mol·L-1. Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation.
袁 燕1，张 薇1，戴建辉1，刘晓芳1，王红斌1，Colin J Barrow2, 杨文荣2 . 荧光光谱法研究蒜头果蛋白与金属离子的相互作用 [J]. 光谱学与光谱分析, 2014, 34(12): 3297-3300.
YUAN Yan1, ZHANG Wei1, DAI Jian-hui1, LIU Xiao-fang1, WANG Hong-bin1, Colin J Barrow2, YANG Wen-rong2 . Studies on the Interaction between Malanin and Metal Ions by Fluorescence Spectra Methods. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(12): 3297-3300.
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