Abstract：A method for simultaneous determination of arsanilic, nitarsone and roxarsone(ROX) residues in foods of animal origin was developed by accelerated solvent extraction-liquid chromatography-atomic fluorescence spectrometry (ASE-LC-AFS). The ultrasound centrifugation extraction and accelerated solvent extraction were compared, and the accelerated solvent extraction conditions, namely the proportion of the extraction solvent, the extraction temperature, extraction time and extraction times, were optimized. The operating conditions of LC-AFS and the mobile phase were optimized. Under the optimal conditions, the calibration curves for ASA , NIT and ROX were linear over the concentration range of 0～2.0 mg·L-1 and their correlation coefficients were 0.999 2～0.999 8. The detection limits of ASA, NIT and ROX were 2.4, 7.4 and 4.1 μg·L-1 respectively. The average recoveries of ASA, NIT and ROX from two samples spiked at three levels of 0.5，2，5 mg·kg-1 were in the ranges of 87.1%～93.2%, 85.2%～93.9%, and 84.2%～93.7% with RSDs of 1.4%～4.6%, 1.2%～4.2%, and 1.1%～4.5%, respectively. This method possesses the merits of convenience and good repeatability, and is a feasible method for analysis of ASA,NIT and ROX in foods of animal origin.
Key words：Accelerated solvent extraction;Liquid chromatography;Atomic fluorescence spectrometry;Food of animal origin;Arsanilic;Nitarsone;Roxarsone;Residues
[1] Laster C P, Hoerr F J, Bilgili S F, et al. Poultry Science, 1999, 78(2): 197. [2] XIAO Ya-bing，WEI Wei，GAO Jian-hui，et al(肖亚兵，韦 玮，高健会，等). Journal of Instrumental Analysis(分析测试学报)，2008, 27 (9) : 964. [3] XIAO Ya-bing，GAO Jian-hui，ZENG Yu，et al(肖亚兵，高健会，曾 宇，等). Journal of Instrumental Analysis(分析测试学报)，2008, 27(11): 1206. [4] Jackson B P, Bertsch P M. Environ. Sci. Technol., 2001, 35(24): 4868. [5] XIAO Ya-bing，YAN Hua，CHEN Qi-yong(肖亚兵，阎 华，陈其勇). Food Research and Development(食品研究与开发)，2011，32(9)：227. [6] SHANG De-rong，NING Jin-song，ZHAO Yan-fang，et al(尚德荣，宁劲松，赵艳芳，等). Journal of Fisheries of China(水产学报)，2010，34(1)：132. [7] MO Shu-min，LIANG Li-na，CAI Ya-qi(墨淑敏，梁立娜，蔡亚岐). Chinese J. Anal. Chem.(分析化学), 2006, 34(4): 493. [8] XIAO Ya-bing，QIN De-yuan，WANG Wei，et al(肖亚兵，秦德元，王 伟，等). Journal of Instrumental Analysis(分析测试学报)，2011, 30(6): 661. [9] Wei C J，Liu J X. Talanta，2007，73: 540. [10] LIU Jian-jing，YU Hong-xia，YANG Shu-ming(刘建静，于洪侠，杨曙明). Applied Chemical Industry(应用化工)，2008，37(6): 694. [11] HUANG Lian-xi，HE Zhao-huan，ZENG Fang，et al(黄连喜，何兆桓，曾 芳，等). Chinese J. Anal. Chem.(分析化学), 2010，38(9): 1321. [12] LIN Yan-kui，LI Yong，WANG Bing-tao，et al(林燕奎，李 勇，王丙涛，等). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志)，2009，19(9): 1955. [13] Machado M E, Fontanive F C, Oliveira J V, et al. Anal. Bioanal. Chem., 2011, 401(8)：2433. [14] Yu H, Tao Y F, Le T. J. Chromatogr. B，2010，878(21)：1746. [15] LI Wen-hui，SHI Ya-li，GAO Li-hong，et al(厉文辉，史亚利，高立红，等). Journal of Instrumental Analysis(分析测试学报)，2010，29(10)：987.
