原子吸收光谱法测定膜荚黄芪不同器官矿质元素含量

doi:10.3964/j.issn.1000-0593.2008.05.007

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摘要：应用混合酸HNO₃-HClO₄ (4∶1) 在常压微沸条件下对膜荚黄芪根系及茎叶样品进行消解，采用原子吸收光谱法测定了膜荚黄芪不同器官即根及茎叶中五种人体必需矿质元素K，Fe，Zn，Mn和Cu含量，并对结果进行了统计分析与比较。该方法标准曲线相关系数为0.997 3～0.999 9, 加标回收率为92.88%～109.25%, 相对标准偏差(RSD，n=5) 为0.393 5%～3.175 2%。方法简单，结果可靠。结果显示，膜荚黄芪根及茎叶中5种矿质元素含量顺序均为K>Fe>Zn>Mn>Cu。膜荚黄芪不同器官矿质元素含量不同，根中富含Fe，Zn，Cu元素，根内Fe含量是茎叶的1.54倍。茎叶中也含有丰富的矿质元素，特别是K和Mn元素。茎叶中K含量是根的1.63倍，这与黄芪的药效相符合。试验结果将为研究矿质元素在黄芪植株中的分布以及矿质元素含量与黄芪药效相关性提供理论依据。

关键词：膜荚黄芪;原子吸收光谱法;根;茎叶;矿质元素

Abstract：Having digested the root and shoot of Astragalus membranaceus (Fisch.) with the mixture of nitric acid and perchloride acid (4∶1) under the condition of the bolling point and the normal pressure, the contents of the five mineral elements necessary to humanity, potassium, copper, zinc, iron and manganese in root and shoot of Astragalus membranaceus (Fisch.) were determined by flame atomic absorption spectroscopy (FAAS), and the results were analyzed in statistics. The correlative coefficient of the standard curve in this method is 0.997 3-0.999 9, the recovery of standard addition was 92.88%-109.25% and the relative standard deviation (RSD, n= 5) was 0.393 5%-3.175 2%. The method is simple and the results were accurate and reliable. The contents of K, Fe, Zn, Mn, Cu in the root and shoot of Astragalus membranaceus (Fisch.) were compared. The results showed that the sequence of the content of metal elements is as follows in all samples: K>Fe>Zn>Mn>Cu. However, the distribution of elements in shoot and root is not uniform, and the content of Fe, Zn and Cu in root is richer than in shoot. There are abundant Fe, Zn and Cu in root, for example, the content of Fe in root is 1.54 times that in shoot. In addition, there are also abundant mineral elements in the shoot, especially in K and Mn, for example, the content of K in shoot is 1.63 times that in root. The contents of K, Fe, Zn, Mn and Cu in shoot are in agreement with the medical effects of Astragalus membranaceus (Fisch.). The results should provide useful data for investigating the distribution of mineral element in Astragalus membranaceu body and the correlation between the mineral element content and the effect of medicine in Astragalus membranaceus (Fisch.).

Key words：Astragalus membranaceus (Fisch.);Flame atomic absorption spectroscopy;Root;Shoot;Metal element

收稿日期: 2007-06-26 修订日期: 2007-09-29

中图分类号:

O657.3

通讯作者: 梁宗锁 E-mail: liangzs@ms.iswc.ac.cn

引用本文:

王渭玲¹,梁宗锁^{1, 2*},谭勇²,段琦梅¹. 原子吸收光谱法测定膜荚黄芪不同器官矿质元素含量[J]. 光谱学与光谱分析, 2008, 28(05): 1168-1171.
WANG Wei-ling¹,LIANG Zong-suo^1,2*,TAN Yong²,DUAN Qi-mei¹. Determination of Mineral Elements in Different Part of Astragalus Membranaceus(Fisch.) by FAAS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1168-1171.

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