Study on Temperature Measurement and Control for Microfluidic Systems
DAI Jing1,FAN Xiao-feng2,FANG Jin3,XU Zhang-run2＊
1. Information & Control Engineering Faculty, Shenyang Jianzhu University, Shenyang 110168, China 2. Research Center for Analytical Sciences, Northeastern University, Shenyang 110004, China 3. Key Laboratory of Cell Biology for Ministry of Public Health of China, China Medical University, Shenyang 110001, China
Abstract：A technique for temperature control and measurement in microfluidic systems was developed. The technique is easy to be implemented in an analytical lab and the experimental setup is relatively simple and inexpensive. The parts for temperature measurement include a CCD camera, a fluorescence microscope and an image acquisition card. The spatial distribution and the temporal variation of temperature in microfluids were measured. The method is non-invasive and the spatial and temporal resolutions were 0.8 μm and 40 ms, respectively a thermal control device was built using indium-tin-oxide (ITO) coated glass as heater, taking advantage of its transparent quality. The temperature could be controlled with a precision of ±0.1 ℃. The image processing procedure was optimized and the efficacy of the method was demonstrated.
Key words：Temperature measurement;Temperature control;ITO film glass;Microfluidic systems
戴敬1,樊晓峰2,方瑾3,徐章润2*. 微流控芯片系统中测温及控温装置的研制[J]. 光谱学与光谱分析, 2008, 28(01): 148-152.
DAI Jing1,FAN Xiao-feng2,FANG Jin3,XU Zhang-run2＊. Study on Temperature Measurement and Control for Microfluidic Systems. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(01): 148-152.
[1] WANG Shi-li, FANG Zhao-lun (王世立，方肇伦). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000,20(2): 143. [2] El-Ali J, Perch-Nielsen I R, Poulsen C R, et al. Sens Actuators A,2004, 110: 3. [3] Smith C R, Sabatino D R, Praisner T J. Exp. Fluids,2001, 30: 190. [4] Chaudhari A M, Woudenberg T M, Albin M, et al. J. Microelectromech Syst, 1998, 7: 345. [5] Liu J, Enzelberger M, Quake S. Electrophoresis,2002,23: 153. [6] Ross D, Gaitan M, Locascio L E. Anal. Chem.,2001, 73: 4117. [7] Mao H, Yang T, Cremer P S. J. Am. Chem. Soc.,2002, 124: 4432. [8] Mao H, Holden M A, You M, et al. Anal. Chem.,2002, 74: 5071. [9] Khandurina J, Mcknight T E, Jacobson S C, et al. Anal. Chem.,2000, 72: 2995. [10] Poser S, Schulz T, Dillner U, et al. Sens. Actuators A, 1997, 62: 672. [11] Woolley A T, Hadley D, Landre P, et al. Anal. Chem., 1996, 68: 4081. [12] Lao A I K, Lee T M H, Hsing I M, et al. Sens. Actuators A,2000, 84: 11. [13] HUANG Chong, OUYANG Yan-dong, WU Yong -jun, et al(黄 , 欧阳艳东，吴永俊，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(5): 637. [14] WANG Gang, LIU Hong-yu, ZHAO Chao, et al(王 刚, 刘宏宇，赵 超，等). Liquid Crystals and Displays(液晶与显示), 1999, 14: 23. [15] LU Xiao-li, YANG Yu-lan, XUE Chang-li, et al(陆晓莉, 杨玉兰, 薛长利, 等). Liquid Crystals and Displays(液晶与显示),2002, 17: 209. [16] Sakakibara J, Adrian R J. Exp. Fluids, 1999,26: 7. [17] Coolen M C J, Kieft R N, Rindt C C M, et al. Exp. Fluids, 1999,27: 420. [18] Dai J, Guan Y X, Wang S L, et al. Anal. Bioanal. Chem.,2005, 381: 839.
