Infrared Spectroscopic Study on Storage Substance Mobilization of Crop Seeds in Germination
LIU Gang1,TAN Li-ping1,DONG Qin2
1. College of Physics and Electronic Information, Yunnan Normal University, Kunming 650092, China 2. Center of Experiment, Yunnan University, Kunming 650092, China
Abstract：The traditional method to study the storage substance mobilization of seeds during the process of germination is chemical extraction, which is troublesome and time consuming. In the present paper, Fourier transform infrared spectroscopy was used to study the storage substance mobilization of legume and cereal seeds in germination. The spectral results show that legume seeds (soybean, pea and broad bean) exhibit similar remarkable changes in the region from 1 000 to 1 200 cm-1 in germination, which suggest that the storage carbohydrates in endosperm of legume seeds are mobilized during the process of germination. Notable changes were observed on amide bands of protein and lipid band in the spectra of germinating cereal seeds (rice, wheat and barley), indicating that storage proteins and lipids in cotyledon were utilized during the process of cereal seed germination. The results suggest that vibrational spectroscopy has the advantages of rapidress and convenience in the study of crop seeds and seed physiology.
刘刚1,谭丽萍1,董勤2. 农作物种子发芽时贮藏物质动员的红外光谱研究[J]. 光谱学与光谱分析, 2008, 28(01): 80-83.
LIU Gang1,TAN Li-ping1,DONG Qin2. Infrared Spectroscopic Study on Storage Substance Mobilization of Crop Seeds in Germination. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(01): 80-83.
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