Research on the DNA Condensation Properties by Ultraviolet Spectrum
ZHANG Jing1, HU Lin2*, LIU Yan-hui2, ZHANG Bi-cheng1
1. College of Life Science, Guizhou University, Guiyang 550025, China 2. Soft Condensed Matter Lab, College of Science, Guizhou University, Guiyang 550025, China
Abstract：In order to explore the property and mechanism of DNA condensation, the authors studies the effect of different concentrations of spermine(SPM), spermidine(SPD) and hexamminecobalt(Ⅲ) chloride on the ultraviolet(UV) absorption value of calf thymus DNA by UV spectrophotometer from the macro. The results showed that SPD and SPM interaction with the DNA had the similar characteristics in UV spectrum. The UV absorption value of calf thymus DNA at 260 nm appeared increased-decreased-increased with the increasing the concentration of SPD and SPM. The value of hexamminecobalt(Ⅲ) chloride system showed decreased and then increased, suggesting that SPM, SPD and hexamminecobalt(Ⅲ) chloride could make DNA condense, but in different ways. Relatively, hexamminecobalt(Ⅲ) chloride was more sensitive than SPD and SPM. The result of UV spectral analysis coincided with recent single-molecule experimental results.
张 婧1，胡 林2*，刘艳辉2，张碧程1 . DNA凝聚性质的紫外光谱研究[J]. 光谱学与光谱分析, 2012, 32(05): 1306-1309.
ZHANG Jing1, HU Lin2*, LIU Yan-hui2, ZHANG Bi-cheng1 . Research on the DNA Condensation Properties by Ultraviolet Spectrum . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(05): 1306-1309.
[1] Smith T A. Annual Review of Plant Physiology, 1985, 36: 117. [2] Galston A W, Kaur-Sawhney R. Plant Physiol., 1990, 94(2): 406. [3] Lusser A, Kadonaga J T. Nature Methods, 2004, 1(1): 19. [4] ZHOU Li-hong, WANG Na, YU Xiao-qi(周立宏, 王 娜, 余孝其). Progress in Chemistry(化学进展), 2007, 19(12): 1909. [5] RAN Shi-yong, WANG Xiao-ling, FU Wen-bo(冉诗勇, 王晓玲, 付文博). Chinese Science Bulletin(科学通报), 2007, 52(14): 1615. [6] LI Zheng-ping, LI Yan-kun, WANG Yu-cong(李正平,李艳坤,王愈聪). Journal of Hebei University(Natural Science Edition)(河北大学学报·自然科学版)，2004, 24(6): 611, 627. [7] PAN Hua, ZHANG Jian, ZHANG Gao-yong(潘 华, 张 剑, 张高勇). Chinese Journal of Applied Chemistry(应用化学)，2007, 24(11): 1250. [8] LIN Zhang, WANG Chen, FENG Xi-zeng(林 璋, 王 琛, 冯喜增). Journal of Chinese Electron Microscopy Society(电子显微学报), 1999, 18(2): 106. [9] PAN Zu-ting, YANG Dai-ling, MENG Fan-chang, et al(潘祖亭, 杨代菱, 孟凡昌, 等). Analytical Chemistry Experiment(分析化学实验). Beijing: Higher Education Press(北京: 高等教育出版社), 2001. [10] Ouameur A A, Tajmir-Riahi H A. J. of Biological Chemistry, 2004, 279(40): 42041. [11] Jing D W, Zhang J, Ma L J, et al. Colloid and Polymer Science, 2004, 282: 1089. [12] Vrána O, Brabec V. Bioelectrochem. Bioene., 1988, 19: 145. [13] Williams D H, Fleming I. Spectroscopic Methods in Organic Chemistry(有机化学中的光谱方法). Beijing: Peking University Press(北京：北京大学出版社), 2001. 1. [14] Baigl D, Yoshikawa K. Biophysical Journal, 2005, 88: 3486. [15] Besteman K, Zevenbergen M A G, Lemay S G. Physical Review E, 2005, 72: 061501. [16] Besteman K, Hage S, Dekker N H, et al. Physical Review Letters, 2007, 98: 058103. [17] Besteman K, Van Eijk K, Lemay S G. Nature Physics, 2007, 3: 641.
