Analysis of the EEM Fluorescence Spectra of Effluent from Anaerobic Methane Reactor during the Inhibition Process
LI Wei-hua1, 2, SHENG Guo-ping1*, LU Rui1, ZHAO Quan-bao1, YU Han-qing1
1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China 2. School of Environment and Energy Engineering, Anhui Institute of Architecture Industry，Hefei 230022, China
Abstract：The effluents from the anaerobic methane reactor were characterized by the EEM fluorescence spectra. The EEM fluorescence spectra and relative concentration of the main components were acquired by PARAFAC models. Results show that the main components of the effluents from the uninhibited reactor were protein, coenzyme NADH and F420. When the methane-producing reaction was inhibited, the production rate of methane decreased rapidly. Correspondingly, the fluorescence of F420 disappeared and the main components of the reactor were protein and NADH. The fluorescence intensity scores of protein from the diluted sample were remarkably correlated to the protein determination by chemical analysis and the correlation efficient was 0.93. The fluorescence intensity scores of F420 were correlated to the production rate of methane, which implicated that coenzyme F420 could be used to reflect the activity of methane production in a certain bioreactor. This work provides a new method to monitor the operation status of anaerobic methane-producing reactor.
李卫华1, 2，盛国平1*，陆 锐1，赵全保1，俞汉青1 . 厌氧产甲烷受抑制过程的三维荧光光谱解析[J]. 光谱学与光谱分析, 2011, 31(08): 2131-2135.
LI Wei-hua1, 2, SHENG Guo-ping1*, LU Rui1, ZHAO Quan-bao1, YU Han-qing1 . Analysis of the EEM Fluorescence Spectra of Effluent from Anaerobic Methane Reactor during the Inhibition Process . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(08): 2131-2135.
[1] Farabegoli G, Hellinga C, Heijnen J J, et al. Water Research, 2003, 37: 2732. [2] Arunachalam R S, Shah H K, Ju L K. Water Research, 2005, 39(7): 1205. [3] YIN Xiao-bo, LIAN Li-wen, XU Jie-quan, et al(尹小波, 连莉文, 徐洁泉, 等). China Biogas(中国沼气), 1998, 16(3): 8. [4] TANG Yi, HU Ji-cui(唐 一, 胡纪萃). China Biogas(中国沼气), 1990, 8(1): 11. [5] Pons M N, Bonté S L, Potier O. Journal of Biotechnology, 2004, 113(1-3): 211. [6] Baker A. Environmental Science & Technology, 2002, 36(7): 1377. [7] Sheng G P, Yu H Q. Water Research, 2006, 40(6): 1233. [8] Wang Z W, Wu Z C, Tang S J. Water Research, 2009, 43(6): 1533. [9] CHEN Mao-fu, WU Jing, Lü Yan-li, et al(陈茂福, 吴 静, 律严励, 等). Acta Optica Sinica(光学学报), 2008, 28(3): 578. [10] Bro R. Chemometrics and Intelligent Laboratory Systems, 1997, 38(2): 149. [11] LI Hong-bin, LIU Wen-qing, ZHANG Yu-jun, et al(李宏斌，刘文清，张玉钧，等). Journal of Atmospheric and Environmental Optics(大气与环境光学学报), 2006, 11(3): 216. [12] WANG Zhi-gang, LIU Wen-qing, LI Hong-bin, et al(王志刚，刘文清，李宏斌，等). Journal of Environmental Sciences(环境科学学报), 2006, 26(2): 275. [13] Li W H, Sheng G P, Liu X W, et al. Water Research, 2008, 42(12): 3173. [14] Fang D M, Wu H L, Ding Y J, et al. Talanta, 2006, 70: 58.
