Abstract：The multicolour three-photon resonant photoionization spectra and high-time-resolved laser spectrum of UI were measured with a setup composed of a Nd∶YAG-laser(532 nm, operated at 10 Hz)-pumped pulsed tunable dye laser system, a time-of-flight mass spectrometer, including micro-channel plate components, ns-oscilloscope, boxcar integrator, and so on. Creative inventions of this paper are for the first time by laser-induced quantum population of the graphic method, the causes for single-colour and two-colour three-photon resonant photoionization spectra peak were given in the three-colour three-photon resonant photoionization experiment;The question how to avoid producing single-colour and two-colour three-photon resonant photoionization spectra peak was solved, That is, how to solve the problem to avoid “false peaks”，so that multicolour three-photon resonant photoionization purity was raised remarkably; On this basis, not only in close proximity to energy level position with just a difference 0.642 cm-1, the isotopes A and B of uranium, which are difficult to distinguish, were well resolved, but the two excited state lifetime values were obtained respectively. This technology is not limited to uranium spectrum, but more importantly, it’s versatile. The new methods and technologies of basic research can be expanded to samples of biological and medical research fields with laser detecting and analysis.
Key words：High-time-resolved laser spectrum;Selective excitation;Multicolour three-photon;Resonant photoionization;Excited state lifetime
史桂珍，杜 海，葛辽海，田 宇，黄茂成，王文韵 . 可扩展到生物医药激光检测的新方法和新技术[J]. 光谱学与光谱分析, 2011, 31(07): 1842-1846.
SHI Gui-zhen, DU Hai, GE Liao-hai, TIAN Yu, HUANG Mao-cheng, WANG Wen-yun . New Methods and Technologies Expandable to the Laser Detection of Biological and Medical Samples. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(07): 1842-1846.
[1] SHI Gui-zhen, DU Hai, WANG Lan, et al(史桂珍，杜 海，王 岚，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(1)：5. [2] SHI Gui-zhen, DU Hai, WANG Shu-bin, et al. J. Opt. Soc. Am. B, 2000, 17(1)：103. [3] ZHAI Li-hua, DENG Hu, WEI Guan-yi, et al(翟利华，邓 虎，韦冠一，等). Journal of Chinese Mass Spectrometry Society(质谱学报)，2008，29(增刊)：229. [4] YIN Yan-bing，JIANG Hai-yan，MAO Xue，et al(尹彦冰，姜海燕，毛 雪，等). Journal of Molecular Science(分子科学学报)，2009，25(5)：365. [5] WANG Yong，XIA Bo，DENG Xiao-chun，et al(王 勇，夏 博，邓晓春，等). Journal of Chinese Mass Spectrometry Society(质谱学报)，2009，30(2)：78. [6] 刘淑莹，王曦烨，宋凤瑞，等. 中国专利：CN101710097A，2010-05-19. [7] 刘志强，鲁 林，宋凤瑞，等. 中国专利：CN101644694，2010-02-10.
