Abstract:The fluorescence excitation spectra and absorption spectra of six kinds of erythrosine solutions with concentrations of 10, 20, 30, 40, 50 and 60 μg·mL-1 were experimentally measured. It was found that the fluorescence excitation peaks are both located at 530 nm significantly when the concentrations of erythrosine solutions are 10 and 20 μg·mL-1. However, the linetype saltation of fluorescence excitation spectrum occurs as the concentration of erythrosine solution is above 30 μg·mL-1. The valley is located at 530 nm and two new peaks appear at both flanks of the valley. Compared with fluorescence excitation spectra, the absorption spectra of erythrosine solutions are without saltation and the peaks are all located at 530 nm. According to calculations and a series of contrast experiments, it was demonstrated that the absorption characteristic of erythrosine and the spectral measurement mode conspire to cause the saltation of fluorescence excitation spectra. The results can provide guidance for further research on physical and chemical properties of erythrosine, and offer help and reference for study on saltation behavior in fluorescence excitation spectra and improvement in spectral measuring mode.
[1] CHEN Guo-qing, WU Ya-min, WEI Bai-lin, et al(陈国庆,吴亚敏,魏柏林,等). Acta Physica Sinica(物理学报), 2010, 59(7): 5100. [2] Wang X M, Wu H L, Nie J F, et al. Chinese Journal of Analytical Chemistry, 2009, 37(6): 811. [3] WEI Bai-lin, CHEN Guo-qing, WANG Jun, et al(魏柏林,陈国庆,王 俊,等). Journal of Optoelectronics·Laser(光电子·激光), 2009, 20(12): 1618. [4] Jana S, Jana T, Pavel M. Food Chemistry, 2009, 117(3): 491. [5] Rita Y, Rachad S, Bilal N, et al. Food Chemistry, 2009, 115(1): 304. [6] Marcelo F P, María S D N, María E C, et al. Talanta, 2006, 69(5): 1265. [7] Tohru S, Hiroto I, Masataka H. Talanta, 2009, 79(2): 177. [8] Rodríguez N, Ortiz M C, Sarabia L A, et al. Analytica Chimica Acta, 2010, 657(2): 136. [9] Sahar A, Boubellouta T, Lepetit J, et al. Meat Science, 2009, 83(4): 672. [10] Natalia B, Roman U, Konstantin V, et al. Medical Laser Application, 2009, 24(4): 247. [11] Manisha S, Renu S, Nivedita K G. Analytical Biochemistry, 2009, 394(2): 186. [12] LU Lu, SU Rong-guo, HU Xu-peng, et al(卢 璐,苏荣国,胡序朋, 等). Chinese Journal of Lasers(中国激光), 2007, 34(8): 1115. [13] SHANG Li-ping, YANG Ren-jie(尚丽平,杨仁杰). In-Situ Fluorescence Spectrum Technology and Its Application(现场荧光光谱技术及其应用). Beijing: Science Press(北京:科学出版社),2009. 6. [14] XU Jin-gou, WANG Zun-ben(许金钩,王尊本). Fluorescence Analysis(荧光分析法). Beijing: Science Press(北京:科学出版社),2006. 7.