Resonance Raman Spectra of Single Red-Cell from Human Blood
YAN Xun-ling1, DONG Rui-xin1，2 , WANG Qiu-guo1
1. Physics Department of Liaocheng University, Liaocheng 252059，China 2. University of Electronic Science and Technology of China, Chengdu 610054，China
Abstract：Resonance Raman spectra of single redcell from human blood are presented by different laser sources. It is reported that there is 1 002 cm-1 line of insensitive conformation of phenylalanine aromatic ring stretching and 1 620 cm-1 line of C—N breathing stretching band of pyrrole ring，which cause strong and sharp resonance lines when eｘcited by 782 nm laser source. They are weaker and the intensity of other lines of high wave number is large and clear when excited by 514 nm laser source. But the intensity of lines of low wave number is strong and clear when eｘcited by 782 nm laser source. At the same time, the authors got Raman spectra lines at different times after taking blood under the same laser source. When using 782 nm laser source, there is no difference except for 1 601 cm-1. There are a lot of weakened lines and lines shifting about 4-10 cm-1 toward low wave number. The results indicate that Raman spectra may offer the experimental basis for studying structure, function and variability of single red-cell.
闫循领1，董瑞新1，2，王秋国1 . 人血单个红细胞的共振拉曼光谱研究[J]. 光谱学与光谱分析, 2004, 24(05): 576-578.
YAN Xun-ling1, DONG Rui-xin1，2 , WANG Qiu-guo1 . Resonance Raman Spectra of Single Red-Cell from Human Blood . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 576-578.
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