Revealing the Cell Structure and Formation of Bamboo with Confocal Raman Microscopy
LI Xiao-li1, ZHOU Bin-xiong1, ZHANG Yi2, YAO Yan-ming3, HE Yong1*
1. College of Biosystems Engineering and Food Science，Zhejiang University，Hangzhou 310058，China 2. Department of Tea Science, Zhejiang University, Hangzhou 310058, China 3. Institute of Harbor Coastal and Nearshore Engineering，Zhejiang University，Hangzhou 310058，China
Abstract：Parenchyma cell (PAC), transition tissue between parenchyma cell and fiber cell (TC) and fibre cell (FC) of bamboo were studied by confocal Raman microscopy in this paper. Partial least squares regression was applied to establish a quantitative differentiation model for the three types of cells. The result showed that the determination coefficients (R2) of calibration and validation were respectively 0.810 and 0.800, and the root mean square error (RMSE) were respectively 0.323 and 0.332. What’s more, three raman bands of 1 095, 1 319 and 1 636 cm-1, verified to the characteristic peaks of pectin, hemicellulose and lignin, were found to be the important bands for the differentiation. Subsequently, these three raman bands were used to establish a multiple linear regression (MLR) model, and the determination coefficients(R2) of calibration and validation of the model were respectively 0.644 and 0.643, and the root mean square error (RMSE) were respectively 0.442 and 0.443. This result showed that there existed obvious difference among the three types of cells in these three raman bands. Finally, the raman spectral signal processed by wavelet transform to eliminate baseline were used to chemical imaging analysis. These results showed a rather large microfibril angle between cellulose fibrils and fibre axis, which contributed to higher modulus and hardness of cells. Hemicellulose and cellulose have similar distribution in the raman chemical image, due to the connection of hemicellulose and cellulose microfiber through hydrogen bond and the closely combination under the action of van der Waals force. The cell corners (CC) and compound middle lamella (CML) were heavily lignified, and a gradual decrease of lignification from the outer layer to the inner layer of the three cells indicate that lignification was first occurred at the CC and CML, and the lignification was not fully completed.
李晓丽1，周斌雄1，张 怡2，姚炎明3，何 勇1* . 基于共聚焦显微拉曼光谱的毛竹细胞结构和成分研究 [J]. 光谱学与光谱分析, 2016, 36(02): 413-418.
LI Xiao-li1, ZHOU Bin-xiong1, ZHANG Yi2, YAO Yan-ming3, HE Yong1* . Revealing the Cell Structure and Formation of Bamboo with Confocal Raman Microscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 413-418.
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