Effects of Different Salinity Stress on K, Na Content and Relevant Genes Expression in Leaves of Chenopodium Album in Xinjiang
YAO Shi-xiang, CHEN Sha-sha, XU Dong-sheng, LAN Hai-yan*
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Abstract：As soil salinity constitutes a major threat to agriculture in the world, to cope with this problem, much emphasis has been focused on the response mechanism of halophytes or salt-tolerant species to salinity stress. In the present study, C. album was treated with long-term NaCl and KCl stress, then several parameters were assayed in leaves as follows: inductive1y coupled p1asma atomic emission spectrometry (ICP-AES) was employed to measure the K, Na content; semi-quantitative RT-PCR was used to investigate the expression level of three genes which were related to ion transport on the vacuolar membrane-NHX, VP1 and VAP-C. In addition, the mechanism for the effect of different salinity on K, Na content was preliminarily discussed. The results were as follows: (1) Under lower concentration of NaCl stress, C. album had a preferential uptake of potassium and exclusion of sodium, and thus maintained a low concentration of sodium in cells of the leaf; (2) Under higher concentration of NaCl (300 mmol·L-1), C. album was able to tolerate excessive amounts of sodium in cells and kept the higher K/Na ratio in cytoplasm by compartmentalizing Na ions into the vacuole via ion transporter system located on vacuole membrane, (3) Much K ions and total ions (including sodium and potassium) may be responsible for, at least partial, the intolerance of C. album to high concentration of KCl. In conclusion, C. album could tolerate high concentration of NaCl stress, accompanied by high ability to accumulate Na+ in leaves. These results could contribute to the further investigation of this promising species, e.g. amending saline soil.
Key words：Chenopodium album;Salt-tolerant plant species;K;Na content;ICP-AES;Genes related to ion transport
姚世响，陈莎莎，徐栋生，兰海燕* . 不同盐胁迫对新疆耐盐植物藜叶片钾、钠元素含量及相关基因表达的影响 [J]. 光谱学与光谱分析, 2010, 30(08): 2281-2284.
YAO Shi-xiang, CHEN Sha-sha, XU Dong-sheng, LAN Hai-yan* . Effects of Different Salinity Stress on K, Na Content and Relevant Genes Expression in Leaves of Chenopodium Album in Xinjiang . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(08): 2281-2284.
