FTIR Spectroscopic Characterization of Material Composition in Leaf of Hevea Brasiliensis Seedlings Under Potassium and Magnesium Deficiency
XUE Xin-xin, WANG Wen-bin, LUO Xue-hua, ZHANG Yong-fa, ZHAO Chun-mei
Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences，Soil and Fertilizer Research Center，Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs，Ministry of Agriculture and Rural Affairs-State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Haikou 571101, China
Abstract：Nutrient deficiency is a vital limiting factor for crop yield and quality improvement. It has restricted the yield and quality of natural rubber for a long time because of the depletion of soil potassium (K) and magnesium (Mg) and their low plant availability in the Laterite soil of rubber plantations, South China. Rubber seedlings of “CATAS 7-33-97” was chosen as test material, the material composition characteristics of rubber leaves under K and Mg deficiency were studied by Fourier transform infrared spectroscopy (FTIR). The changes and differences of FTIR characteristic peaks of leaves were analyzed, and the material compositions and structures were discussed. It would provide some physiological evidence of rubber plants subjected to K and Mg deficiency stress. The results showed that: (1) the characteristic peaks of 1 554, 1 519 and 1 075 cm-1 disappeared due to K deficiency, implying that the structure of amide Ⅱ band, phenolic compounds and carbohydrate were broken. The relative absorbance of all characteristic peaks was increased compared with normal treatments, suggesting that protein and carbohydrate were accumulated in leaf to reduce their translocation efficiency. (2) The absorption peak at 1 554 cm-1 shifted 6 cm-1 to high frequency as 1 560 cm-1 when the plant was subject to Mg deficiency, which indicated that the structure of the protein amide Ⅱ band was changed. Meanwhile, Mg deficiency decreased the relative absorbance in the range of 1 550~1 350 cm-1, implying that Mg deficiency decreased the contents of polysaccharides and lipid compounds of the leaf cell wall. The three absorption peaks in the wave number of 1 550~1 350 cm-1 were more sensitive to Mg deficiency than other absorption peaks, indicating that the wave band could better represent the magnesium nutrition status of rubber plant leaves. (3) The relative absorbance and intensity of each absorption peak decreased obviously at both K and Mg deficiency. Absorption peaks at 1 554, 1 519 and 1 075 cm-1 disappeared similar to that K deficiency, which indicated that the chemical structure of protein amide Ⅱ band, phenols and carbohydrates was destroyed. Meanwhile, the absorption peak of 1 057 cm-1 shifted to the high frequency direction by 11 cm-1 to 1 068 cm-1, implying the molecular structure of pectin polysaccharides changed in rubber leaves. In conclusion, K deficiency caused a large accumulation of protein and carbohydrates in rubber leaves, and magnesium deficiency caused the decrease of polysaccharide and lipid compounds in the leaf cell wall. However, the contents of protein, lipid and carbohydrates decreased significantly when the plant was subjected to both K and Mg deficiency. The results showed that FTIR technology was feasible for qualitative analysis of the material components of rubber leaves under nutrient deficiency and could provide new ideas and methods for studying the mechanism of K and Mg nutrition and physiological metabolism of the rubber trees.
薛欣欣，王文斌，罗雪华，张永发，赵春梅. 钾镁胁迫对橡胶幼苗叶片物质成分影响的FTIR表征[J]. 光谱学与光谱分析, 2022, 42(01): 74-79.
XUE Xin-xin, WANG Wen-bin, LUO Xue-hua, ZHANG Yong-fa, ZHAO Chun-mei. FTIR Spectroscopic Characterization of Material Composition in Leaf of Hevea Brasiliensis Seedlings Under Potassium and Magnesium Deficiency. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 74-79.
[1] LI Tao, LI Qin, CHEN Lin-yang, et al(李 涛, 李 芹, 陈林杨, 等). Journal of Yunnan Agricultural University·Natural Science(云南农业大学学报·自然科学), 2017, 32(2): 303.
[2] CHEN YONG-chuan, YANG Chun-xia, ZHAO Zhi-ping, et al(陈永川, 杨春霞, 赵志平, 等). Chinese Journal of Ecology(生态学杂志), 2012, 31(4): 954.
[3] WANG Da-peng, WANG Xiu-quan, CHENG Jing, et al(王大鹏, 王秀全, 成 镜, 等). Chinese Journal of Tropical Agriculture(热带农业科学), 2013, 33(9): 22.
[4] XUE Xin-xin, WU Xiao-ping, LUO Xue-hua, et al(薛欣欣, 吴小平, 罗雪华, 等). Chinese Journal of Tropical Crops(热带作物学报), 2019, 40(8): 1507.
[5] HUA Yuan-gang, BEI Mei-rong, LIU Hai-lin, et al(华元刚, 贝美容, 刘海林, 等). Chinese Journal of Tropical Crops(热带作物学报), 2019, 40(4): 623.
[6] Gransee A, Führs H. Plant and Soil, 2013, 368(1): 5.
[7] DU Zhong-jie, LIN Dian, XU Jie, et al(杜忠杰, 林 电, 许 杰, 等). Guangdong Agricultural Sciences(广东农业科学), 2011, 38(11): 73.
[8] LI Peng-yi, ZHENG Mei-xuan, LIANG Xiu-qiu, et al(李彭怡, 郑美漩, 梁秀球, 等). Chinese Journal of Tropical Agriculture(热带农业科学), 2019, 39(8): 1.
[9] WENG Shi-fu(翁诗甫). Fourier Transform Infrared Spectrum Analysis(傅里叶变换红外光谱分析). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2010.
[10] Jebsen C, Norici A, Wagner H, et al. Physiologia Plantarum, 2012, 146(4): 427.
[11] Heiko W, Christian J. Journal of Phycology, 2019, 55: 543.
[12] Butler H J, Adams S, Mcainsh M R, et al. Vibrational Spectroscopy, 2017, 90: 46.
[13] WANG Sheng-feng, LIU Yun-xia, GAO Li-li, et al(王盛峰，刘云霞，高丽丽，等). Journal of Plant Nutrition and Fertilizers(植物营养与肥料学报), 2014, 20(4): 1005.
[14] YAO Yu-jie, JIANG Cun-cang(姚宇洁, 姜存仓). South China Fruits(中国南方果树), 2016, 45(6): 10.
[15] HAO Yan-shu, JIANG Cun-cang, WANG Xiao-li, et al(郝艳淑, 姜存仓, 王晓丽, 等). Acta Agronomica Sinica(作物学报), 2011, 37(11): 2094.
[16] HU Wen-shi, LU Zhi-feng, MENG Fan-jin, et al(胡文诗, 陆志峰, 孟凡金, 等). Plant Physiology Journal(植物生理学报), 2019, 55(6): 774.
[17] SHI Ji-yong, ZOU Xiao-bo, ZHAO Jie-wen, et al(石吉勇, 邹小波, 赵杰文, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2011, 27(8): 283.
