Abstract：A new spectrofluorimetric method for the determination of trace amount of Fe(Ⅲ) has been developed. This method is based on the fluorescence quenching of 2,4-dichro-phenylfluorone due to the formation of complex Fe(Ⅲ)-DCIPF. In pH 5.2-5.9 buffer solution, Fe(Ⅲ) and DCIPF react on each other to form a red complex. Its composition was established by method of continuous variations and molar-ratio as Fe(Ⅲ)∶DCIPF =1∶4, the excitation and emission wavelengths were found to be 282 and 560 nm, respectively. There is a linear relationship in the range 4-24 ng·mL-1 for Fe(Ⅲ). This method is simple and rapid, and has been applied to the determination of trace iron in Chinese herbal medicine and tea samples with satisfactory results．
代 钢，敖登高娃. 2，4-二氯苯基荧光酮-Triton X-100荧光熄灭法测定中草药、茶叶中的微量Fe [J]. 光谱学与光谱分析, 2004, 24(07): 848-850.
DAI Gang, AODENG Gao-wa . Determination of Iron(Ⅲ) in Chinese Herbal Medicine and Tea Based on Fluorescence Quenching of 2,4-Dichro-Phenylfluorone . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 848-850.
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