Studies on Human Breast Cancer Tissues with Raman Microspectroscopy
YU Ge1, XU Xiao-xuan1, NIU Yun2, WANG Bin1, SONG Zeng-fu3,ZHANG Chun-ping1*
1. Photonics Center, Institute of Physics, Nankai University, Tianjin 300071, China 2. Tumour Hosptial, Tianjin Medical University, Tianjin 300060, China 3. College of Physics, Peking University, Beijing 100871, China
Abstract：The microscopic Raman spectra from normal and malignant human breast tissues have been measured and investigated. The spectral differences and changes between normal and malignant breast tissue samples mainly involve: (1) the band from the symmetric stretching modes of PO-2 group in the DNA shifts from 1 082 to 1 097 cm-1 and becomes stronger. The intensity of the symmetric stretching modes of O—P—O at 817 cm-1 in RNA increases greatly. (2) The bands of Amide Ⅰ and Ⅲ at 1 657 and 1 273 cm-1 change to 1 662 and 1 264 cm-1 respectively with their intensity and band width increasing. The peak of the C—O stretching modes in the amino acids shifts to higher wave number. The tryptophane band at 1368 cm-1 almost disappears. (3) Fewer characteristic Raman bands from lipids are observed. These spectral changes indicate that nucleic acids increase in contents relatively, while their conformation changes in cancer tissues. The proteins show various conformations and disorder structures with their molecular hydrogen bonds nearly broken. The contents of lipids decrease obviously. This investigation shows that Raman microspectroscopy is useful to biochemical study and vivo diagnosis of human breast cancers.
于 舸1，徐晓轩1，牛 昀2，王 斌1，宋增福3，张春平1* . 人乳腺癌组织的显微拉曼光谱研究[J]. 光谱学与光谱分析, 2004, 24(11): 1359-1362.
YU Ge1, XU Xiao-xuan1, NIU Yun2, WANG Bin1, SONG Zeng-fu3,ZHANG Chun-ping1* . Studies on Human Breast Cancer Tissues with Raman Microspectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1359-1362.
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