Speciation Analysis of Cadmium in Cadmium-Enriched Plants with SE-HPLC Combined with ICP-MS and ESI-MS
LI Bin1, LIU Li1, WANG Qiu-quan2, YAN Hua2, HUANG Ben-li2
1. Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China 2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Abstract：Different species of ferns (pyrrosia lingua，Lepisorus thunbergianus，Lycopodium japonicum，Elaphoglossum yoshinagae and Woodwardia japonica) were cultivated using different species of cadmium as cultivation solution. The results showed that there were differences in enrichment amounts of cadmium in different parts of plants (amounts in root＞in stem＞in leaves). And it was found that different species of cadmium could induce synthesization of plant-chelated peptides (PCs) with different extents. And then PCs was coordinated with cadmium to decrease the toxicity from cadmium. There are some kinds of peptides ligands found in plants, which are mainly PC3, iso-PC3(βAla) and iso-PC2(βAla). In the present paper, the distribution of cadmium in different parts of ferns was studied after the ferns were intimidated with different species of cadmium. And the species of PCs-Cd coordination compounds were also investigated to explain the detoxification mechanism aroused by it.
李 彬1，刘 丽1，王秋泉2，严 华2，黄本立2 . SE-HPLC/ICP-MS/ESI-MS联用技术用于富镉植物中镉的形态研究[J]. 光谱学与光谱分析, 2010, 30(04): 1096-1100.
LI Bin1, LIU Li1, WANG Qiu-quan2, YAN Hua2, HUANG Ben-li2 . Speciation Analysis of Cadmium in Cadmium-Enriched Plants with SE-HPLC Combined with ICP-MS and ESI-MS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(04): 1096-1100.
[1] Klueppel D, Jakubowski N, Messerschmidt J, et al. J. Anal. At. Spectrom., 1998, 13: 255. [2] Prasad M N V. Analusis, 1998, 26: M25. [3] Zenk M H. Gene, 1996, 179: 21. [4] Leopold I, Guenther D, Neumann D. Analusis, 1998,26: M28. [5] Das A K, Chakraborty R, Cervera M L, et al. Mikrochim. Acta, 1996, 122: 209. [6] Szpunar J, Lobinski R. Pure Appl. Chem., 1999, 71: 899. [7] Szpunar J. Trends Anal.Chem., 2000, 19: 127. [8] Rauser W E. Annu. Rev. Biochem., 1990, 59: 61. [9] Grill E, Loffler S, Winnacker E L, et al. Proc. Natl. Acad. Sci., 1989, 86: 6838. [10] Sneller F E C, Van Heerwaarden L M, Koevoets P L M, et al. J. Agric. Food Chem., 2000, 48: 4014. [11] Grill E, Winnacker E L, Zenk M H. Science, 1985, 230: 674. [12] Delhaize E, Jackson P J, Lujan L D, et al. Plant Physiol., 1989, 89: 700. [13] Leopold I, Gunther D. Fresenius’J. Anal. Chem., 1997, 359: 364. [14] Makarov A, Szpunar J. Analusis, 1998, 26: M44. [15] Hutchens T W, Nelson R W, Allen M H, et al. Biol. Mass Spectrom., 1992, 21: 151. [16] Grese R P, Cerny R L, Gross M L. J. Am. Chem. Soc., 1989, 111: 2835. [17] Vaisar T, Gatlin C L, Turecek F. J. Mass Spectrom., 1995, 30: 1617. [18] Vacchina V, Lobinski R, Oven M, et al. J. Anal. At. Spectrom., 2000, 15: 529. [19] Vacchina V, Polec K, Szpunar J. J. Anal. At. Spectrom., 1999, 14: 1557. [20] Vacchina V, Chassaigne H, Oven M, et al. The Analyst, 1999, 124: 1425. [21] Lobinski R, Szpunar J. Anal. Chim. Acta, 1999, 400: 321.
