Abstract：The interaction of Pb2+ and DNA of fish intestines was investigated using absorption spectrum, fluorescence emission spectrum, and electrophoresis. Ultraviolet absorption spectra indicated that with the addition of Pb2+, the DNA generated obvious hypochromic effect, and the absorption peak at 207 nm of DNA was blue-shifted. The fluorescence emission spectra showed that with the addition of Pb2+, the emission peak of DNA at about 419 nm did not have obvious changes, but the intensity of fluorescence reduced gradually, showing that Pb2+ has quenching effect on the DNA endogenous fluorescence. Pb2+ has 0.8 binding sites to DNA, and the fluorescence quenching of DNA caused by Pb2+ belongs to static quenching. The binding constants of binding sites are 6.08×104 L·mol-1 and 2.82×104 L·mol-1 from the Lineweaver-Burk plot. The experiments of ultraviolet and fluorescence spectra showed that exogenous Pb2+ could cause some changes to the conformation of DNA, but analysis of electrophoresis proved that exogenous Pb2+ at different concentration did not result in DNA breakage.
洪法水，蔡曙明，刘 超，郑 蕾，吕世鹏 . Pb2+对鱼肠DNA光谱特性的影响 [J]. 光谱学与光谱分析, 2005, 25(03): 424-427.
HONG Fa-shui, CAI Shu-ming, LIU Chao, ZHENG Lei, Lü Shi-peng . Effect of Pb2+ on Spectral Characteristics of DNA from Fish Intestines . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 424-427.
[1] Sunderman F W et al. Fed. Proc.，1978, 37(1): 40. [2] Yamane et al(山根靖弘等编著). Environmental Pollutant and Toxicity(环境污染物质与毒性). Chengdu: Sichuan People Press(成都: 四川人民出版社), 1981. 108. [3] Wang J H et al. J.Biol.Chem., 1977, 252: 4175. [4] SUN Ce(孙 册). Chemistry of Life(生命的化学)，1991，11(3)：34. [5] YANG P et al. Acta Chemica Sinica(English Edition), 1989, 47(5): 443. [6] Lu T B et al. Enzyme and Microbial Tech., 1996. 339. [7] Zhang Z X et al. Chinese Science Bulletin, 1986, 31(3): 205. [8] Mao Y et al. Chinese Science Bulletin, 1989, 34(19):1668. [9] Zhou Y Q et al. Biophys. Chemistry, 1994, 51: 81. [10] Zhou Y Q et al. Biochem. J., 1994, 304: 23. [11] HU Xu-ying et al(胡绪英等). Science in China(Series B)(中国科学B辑), 1997, 27(1): 75. [12] WANG Shu-ling et al(王树玲等). Chinese J. Inorg. Chem.(无机化学学报), 2002, 18(7): 8. [13] Marmur J. J. Mol. Biol.，1961, 3：208. [14] Rechmanm M E et al. J. Am. Chem. Soc., 1954, 76：3047. [15] Pyle A M et al. J. Am. Chem. Soc., 1989, 111, 3051. [16] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, XU Jin-gou, WANG Zun-ben(陈国珍，黄贤智，郑朱梓，许金钩，王尊本). Fluorescence Analytical Method(荧光分析法). Beijing: Science Press(北京: 科学出版社), 1990. 502. [17] ZHOU Xin-wen et al(周新文等). Nucleus Technology(核技术), 2002, 25(6): 408. [18] XIANG Li-xin et al(项黎新等). Prog. Biochem. Biophys.(生物化学与生物物理进展), 2001, 28(6): 866.
