Determination of Deoxyribonucleic Acid with Acridine Orange by Resonance Light Scattering Technique
WAN Xin-jun1, LIU Jin-shui2, WANG Lun2*
1. Department of Chemistry, Chaohu College, Chaohu 238000, China 2. College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, China
Abstract：The resonance light scattering (RLS) spectra of acridine orange with deoxyribonucleic acid have been studied. The RLS of acridine orange is greatly enhanced by deoxyribonucleic acid in pH 11. There is a resonance light scattering peak at 324 nm. Under optimal conditions, the linear ranges of the calibration curves are 7.5×10-8-9.8×10-6 g·mL-1 for Calf thymus DNA (ct DNA)，3.1×10-8-7.3×10-6 g·mL-1 for Fish sperm DNA (fs DNA) with detection limits 20.8 ng·mL-1 for ct DNA and 20.5 ng·mL-1 for fs DNA. This method is simple, selectivity and sensitivity has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.
万新军1，刘金水2，王 伦2* . 吖啶橙共振光散射法测定脱氧核糖核酸[J]. 光谱学与光谱分析, 2005, 25(05): 754-756.
WAN Xin-jun1, LIU Jin-shui2, WANG Lun2*. Determination of Deoxyribonucleic Acid with Acridine Orange by Resonance Light Scattering Technique. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(05): 754-756.
