Abstract：The interaction of water-soluble p-(N, N-dimethylaminomethyl)calix[8]arene (CX8) with DNA was studied using adriamycin(ADM) as a probe by fluorescent spectrometry.The effect factors such as, pH of solution, salt effect and unlinking DNA on the spectra were also investigated.The interaction mechanism was proposed.The authors observed that the fluorescence of ADM was quenched by calf thymus DNA, and the fluorescence intensity of DNA-ADM enhanced with the increasing of the CX8 gradually.The result indicates that the strong electrostatic interaction between the oxyphosphate anion of DNA and the CX8 existed.According to the plot of scatchard, the results imply that the influence of CX8 on interaction of DNA-ADM belongs to mixed model, on the one hand, in the condition of neutral and acidity, the oxyphosphate anion of DNA can be neutralized by CX8 partially, which induced the contraction of DNA and the conformational change of DNA, which led to release ADM from DNA partially，on the other hand, the CX8 can also compete the electrostatic sites with ADM directly.
李来生，黄志兵，王宇晓，刘 旭，李艳萍，葛小辉. 荧光光谱法研究对-二甲氨甲基-杯[8]芳烃与DNA相互作用[J]. 光谱学与光谱分析, 2005, 25(07): 1088-1091.
LI Lai-sheng, HUANG Zhi-bing, WANG Yu-xiao，LIU Xu，LI Yan-ping，GE Xiao-hui. Study on the Interaction of Water-Soluble p-(N, N-Dimethylaminomethyl)calix[8]arene with DNA by Fluorescent Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1088-1091.
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