化学发光法测定尿酸

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摘要：利用尿酸对碱性luminol-H₂O₂-Co²⁺化学发光体系具有较强的抑制作用，建立了一种测定尿酸的新方法。方法的线性范围为1.0×10^-10～7.0×10^-6 mol·L^-1，检测限(3σ)为1.1×10^-11 mol·L^-1，RSD 1.9%(n=4，ｃ_s=5.0×10^-8 mol·L^-1)。该法用于人体血清及尿液中尿酸的测定，结果满意。

关键词：化学发光;尿酸;Co²⁺

Abstract：A novel chemiluminescence method for the determination of uric acid based on its inhibitory effect on the catalysis of Co²⁺ for the oxidation of luminol by H₂O₂ is described.The proposed method is simple, very sensitive and inexpensive.The decrease of chemiluminescence intensity is linearly correlated with uric acid concentration.The calibration graph is linear over the range of 1.0×10^-10-7.0×10^-6 mol·L^-1 and the detection limit is 1.1×10^-11 mol·L^-1.The relative standard deviation (RSD) for 5.0×10^-8 is 1.9%(n=4).The method has been successfully applied to the determination of uric acid in human urine and serum samples.

Key words：Chemiluminescence;Uric acid;Co²⁺

收稿日期: 2004-10-26 修订日期: 2005-01-10

中图分类号:

R446.1

通讯作者: 刘二保

引用本文:

刘二保^1*，卫洪清^1，2. 化学发光法测定尿酸[J]. 光谱学与光谱分析, 2005, 25(08): 1213-1215.
LIU Er-bao^1*, WEI Hong-qing^1，2 . Determination of Uric Acid by Chemiluminescence. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1213-1215.

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