Fluorescence Used to Investigate the Sensitivity of Spinach Chloroplast Membrane to Low Intensity Electromagnetic Radiation
XI Gang1, YANG Yun-jing2,LU Hong3
1. Department of Physics, Xi’an University of Technology, Xi’an 710048, China 2. Department of Applied Physics, Northwest A & F University, Yangling 712100, China 3. College of Science, South China Agricultural University, Guangzhou 510642, China
Abstract：A system for studying biological effect of radio frequency electromagnetic field was developed. The system can form an area where electromagnetic wave with large frequency range is well distributed. The strength of electromagnetic wave was measured easily. Electromagnetic wave in the system did not have effect on environment. The sensitivity of spinach chloroplast membrane to low intensity electromagnetic radiation of 300 MHz under power density of 5 mW·cm-2 was studied by the spectral analysis method of fluorescence of 8-anilino-1-naphthalene-sulfonic acid (ANS) and the changes in chlorophyll a (Chla) fluorescence parameters of spinach chloroplast membrane. The result showed that the position of spectrum of ANS fluorescence of spinach chloroplast membrane did not change, but the intensity of ANS fluorescence was obviously increased under the action of electromagnetic radiation with power density of 1-5 mW·cm-2. There was an increase in the intensity of ANS fluorescence with the increase in electromagnetic radiation. The increase of ANS fluorescence of spinach chloroplast membrane showed that low level electromagnetic field induced the decrease in fluidity of chloroplast membrane compared with control experiment. The cause of the change in the fluidity could be related to the polarization of chloroplast membrane under the electromagnetic field. The analysis of Chla fluorescence parameters of spinach chloroplast membrane indicated that low level electromagnetic field of 300 MHz made the fluorescence parameters F0 and FVI／FV decrease, and FV／F0, FV／Fm and ΔFV／T increase. It was showed that low level electromagnetic field caused the change of non-active center of photosystem Ⅱ of spinach chloroplast membrane to active center and the increase in potential active and photochemical efficiency of PSⅡ, and promoted the transmit process of electron in photosynthesis of chloroplast membrane of photosynthesis cell in spinach leaf. The study confirmed that low level electromagnetic field has non-thermal effects on photosynthesis system of spinach chloroplast membrane. The cell in spinach leaf can keep the photosynthesis through the change in heterogeneity of photosystem Ⅱ and adapt to the environment of electromagnetic radiation increase.
习岗1,杨运经2,卢洪3 . 应用荧光探针研究菠菜叶绿体膜对低强度电磁场的辐射敏感性[J]. 光谱学与光谱分析, 2009, 29(07): 1920-1924.
XI Gang1, YANG Yun-jing2,LU Hong3 . Fluorescence Used to Investigate the Sensitivity of Spinach Chloroplast Membrane to Low Intensity Electromagnetic Radiation . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1920-1924.
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