1. Department of Mathematics and Physics, Beijing Institute of Petrochemical Technology, Beijing 102617, China 2. Institute of Physics, Nankai University, Tianjin 300071, China
Abstract：A linear regression model for Raman spectra of human breast tissue was developed with 10 basis spectra which were derived from the spectra of fat, cell cytoplasm, collagen, β-carotene, DNA and cholesterol etc. This model was tested statistically with more than 2 000 spectra collected from both normal and tumor breast tissue samples. The confidence of F-test for the model significance was 1 to all the spectra and the average of multiple determination coefficients measuring the fit goodness was 0.95. The normalized fit coefficients reflected, to a certain extent, the relative quantities of the Raman-active components in tissue represented by the basis spectra. In comparison with normal breast tissue, the coefficients of the cell cytoplasm and DNA basis spectra increased apparently for the tumor one, and the coefficient of fat basis spectrum changed reversely, which was easily understood with the known pathological changes in the tissue. Mapping spectra were also fitted with this model. This work is helpful to understanding the biochemical/morphological changes in the breast tumor tissues and to developing the Raman diagnostic method for breast tumor.
Key words：Breast tissue;Raman spectrum;Basis spectrum;Linear model;Fit coefficients;F test
于舸1,吕爱君1,王斌2,谭恩忠1,高德文1. 乳腺组织的拉曼光谱线性模型研究[J]. 光谱学与光谱分析, 2008, 28(05): 1091-1094.
YU Ge1,Lü Ai-jun1,WANG Bin2,TAN En-zhong1,GAO De-wen1. Study on Raman Linear Model of Human Breast Tissue. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1091-1094.
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